| pubmed-article:8406681 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8406681 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:8406681 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:8406681 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:8406681 | lifeskim:mentions | umls-concept:C0004112 | lld:lifeskim |
| pubmed-article:8406681 | lifeskim:mentions | umls-concept:C0085140 | lld:lifeskim |
| pubmed-article:8406681 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:8406681 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:8406681 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:8406681 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8406681 | pubmed:dateCreated | 1993-11-8 | lld:pubmed |
| pubmed-article:8406681 | pubmed:abstractText | We used the fluorescent pH-sensitive dye 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) to monitor intracellular pH (pHi) in single astrocytes cultured from the forebrain of neonatal rats. When exposed to a nominally CO2/HCO3(-)-free medium buffered to pH 7.40 with HEPES at 37 degrees C, the cells had a mean pHi of 6.89. Switching to a medium buffered to pH 7.40 with 5% CO2 and 25 mM HCO3(-) caused the steady-state pHi to increase by an average of 0.35, suggesting the presence of a HCO3(-) -dependent acid-extrusion mechanism. The sustained alkalinization was sometimes preceded by a small transient acidification. In experiments in which astrocytes were exposed to nominally HCO3(-)-free (HEPES-buffered) solutions, the application and withdrawal of 20 mM extracellular NH4+ caused pHi to fall to a value substantially below the initial one. pHi spontaneously recovered from this acid load, stabilizing at a value approximately 0.1 higher than the one prevailing before the application of NH4+. In other experiments conducted on cell bathed in HEPES-buffered solutions, removing extracellular Na+ caused pHi to decrease rapidly by 0.5. Returning the Na+ caused pHi to increase rapidly, indicating the presence of an Na(+)-dependent/HCO3(-)-dependent acid-extrusion mechanism; the final pHi after returning Na+ was approximately 0.08 higher than the initial value. This pHi recovery elicited by returning Na+ was not substantially affected by 50 microM ethylisopropylamiloride (EIPA), but was speeded up by 50 microM 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS).(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
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| pubmed-article:8406681 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:8406681 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8406681 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8406681 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8406681 | pubmed:issn | 0894-1491 | lld:pubmed |
| pubmed-article:8406681 | pubmed:author | pubmed-author:BoronW FWF | lld:pubmed |
| pubmed-article:8406681 | pubmed:author | pubmed-author:SchlueW RWR | lld:pubmed |
| pubmed-article:8406681 | pubmed:author | pubmed-author:DavisM BMB | lld:pubmed |
| pubmed-article:8406681 | pubmed:author | pubmed-author:BoyarskyGG | lld:pubmed |
| pubmed-article:8406681 | pubmed:author | pubmed-author:RansomBB | lld:pubmed |
| pubmed-article:8406681 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8406681 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:8406681 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8406681 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8406681 | pubmed:pagination | 241-8 | lld:pubmed |
| pubmed-article:8406681 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:8406681 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8406681 | pubmed:articleTitle | Intracellular pH regulation in single cultured astrocytes from rat forebrain. | lld:pubmed |
| pubmed-article:8406681 | pubmed:affiliation | Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510. | lld:pubmed |
| pubmed-article:8406681 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8406681 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8406681 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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