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pubmed-article:8406546pubmed:abstractTextPhosphatidylcholine liposomes have been used as carriers of mannophosphoinositides (PIMs) of mycobacteria to examine their immunological properties. PIMs incorporated in egg phosphatidylcholine (EPC) liposomes elicited both humoral and cell-mediated immune responses in mice. Addition of cholesterol at 43 mole% to PC enhanced the immune responses while the reverse was observed with EPC liposomes bearing negative or positive charge. Liposomes made of dioleoylphosphatidylcholine (DOPC) with cholesterol (43 mole%) proved to be a better immunoadjuvant with this antigen as compared to those made of EPC or dipalmitoylphosphatidylcholine (DPPC). Our results suggest that DOPC liposomes containing cholesterol are better carriers for PIMs-antigen as compared to Freund's incomplete adjuvant.lld:pubmed
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pubmed-article:8406546pubmed:authorpubmed-author:SinghA PAPlld:pubmed
pubmed-article:8406546pubmed:authorpubmed-author:KhullerG KGKlld:pubmed
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pubmed-article:8406546pubmed:articleTitleLiposomes as a carrier for mannophosphoinositide antigens of mycobacteria.lld:pubmed
pubmed-article:8406546pubmed:affiliationDepartment of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh, India.lld:pubmed
pubmed-article:8406546pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8406546pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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