| pubmed-article:8396485 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8396485 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:8396485 | lifeskim:mentions | umls-concept:C0699799 | lld:lifeskim |
| pubmed-article:8396485 | lifeskim:mentions | umls-concept:C0027836 | lld:lifeskim |
| pubmed-article:8396485 | lifeskim:mentions | umls-concept:C0033634 | lld:lifeskim |
| pubmed-article:8396485 | lifeskim:mentions | umls-concept:C1150423 | lld:lifeskim |
| pubmed-article:8396485 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:8396485 | lifeskim:mentions | umls-concept:C0200940 | lld:lifeskim |
| pubmed-article:8396485 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8396485 | pubmed:dateCreated | 1993-10-8 | lld:pubmed |
| pubmed-article:8396485 | pubmed:abstractText | The activity of calcium-independent, calcium-dependent and calcium + phospholipid-dependent (protein kinase C) kinases in cytosol fractions from rat brain glial cells in primary culture and from subcultured astrocytes and in oligodendrocyte-type 2 astrocyte lineage glia has been measured with histone Type IIIS as substrate. Accurate measurement of protein kinase C activity was achieved only after chromatography of glial cytosols on DE-52 anion exchange columns to remove an endogenous inhibitor, identified tentatively as a phosphatase possibly of the protein phosphatase 2A class. The specific activity of protein kinase C in glial cell cytosol increased from 7.5 +/- 0.8 to 37.9 +/- 0.9 nmol32P incorporated/mg protein/10 min with increasing culture age. Protein kinase C activity in glial cytosol was significantly higher when primary cultures were grown in a defined medium lacking serum. Astrocyte-conditioned medium and phorbol esters caused a rapid translocation of glial cell protein kinase C activity from cytosol to membrane compartments. Myelin basic protein and protamine have been compared with histone as substrates for measurement of calcium-independent, calcium-dependent and calcium + phospholipid-dependent kinase activities in glial cytosol. | lld:pubmed |
| pubmed-article:8396485 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396485 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8396485 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396485 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8396485 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396485 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396485 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396485 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8396485 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8396485 | pubmed:issn | 0197-0186 | lld:pubmed |
| pubmed-article:8396485 | pubmed:author | pubmed-author:TurnerRR | lld:pubmed |
| pubmed-article:8396485 | pubmed:author | pubmed-author:RumsbyM GMG | lld:pubmed |
| pubmed-article:8396485 | pubmed:author | pubmed-author:MurphyJ AJA | lld:pubmed |
| pubmed-article:8396485 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8396485 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:8396485 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8396485 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8396485 | pubmed:pagination | 87-94 | lld:pubmed |
| pubmed-article:8396485 | pubmed:dateRevised | 2009-9-29 | lld:pubmed |
| pubmed-article:8396485 | pubmed:meshHeading | pubmed-meshheading:8396485-... | lld:pubmed |
| pubmed-article:8396485 | pubmed:meshHeading | pubmed-meshheading:8396485-... | lld:pubmed |
| pubmed-article:8396485 | pubmed:meshHeading | pubmed-meshheading:8396485-... | lld:pubmed |
| pubmed-article:8396485 | pubmed:meshHeading | pubmed-meshheading:8396485-... | lld:pubmed |
| pubmed-article:8396485 | pubmed:meshHeading | pubmed-meshheading:8396485-... | lld:pubmed |
| pubmed-article:8396485 | pubmed:meshHeading | pubmed-meshheading:8396485-... | lld:pubmed |
| pubmed-article:8396485 | pubmed:meshHeading | pubmed-meshheading:8396485-... | lld:pubmed |
| pubmed-article:8396485 | pubmed:meshHeading | pubmed-meshheading:8396485-... | lld:pubmed |
| pubmed-article:8396485 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8396485 | pubmed:articleTitle | The protein kinase C, calcium-independent and calcium-dependent kinase activities of glial cells in primary culture and subculture. | lld:pubmed |
| pubmed-article:8396485 | pubmed:affiliation | Department of Biology, University of York, England. | lld:pubmed |
| pubmed-article:8396485 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8396485 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8396485 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
