| pubmed-article:8396212 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8396212 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8396212 | lifeskim:mentions | umls-concept:C0006104 | lld:lifeskim |
| pubmed-article:8396212 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:8396212 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:8396212 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:8396212 | pubmed:dateCreated | 1993-10-4 | lld:pubmed |
| pubmed-article:8396212 | pubmed:abstractText | A soluble tripeptidylaminopeptidase has been isolated from human post-mortem cerebral cortex by anion exchange, hydrophobic interaction and size-exclusion chromatography. From gel filtration studies the active enzyme can exist in both high molecular weight (M(r) > 10(6) and smaller forms. The enzyme hydrolyses Ala-Ala-Phe-7-amido-4-methylcoumarin with a pH optimum of around 7.5 and Km of 148 microM. It did not hydrolyse N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin, aminoacyl- or dipeptidyl-7-amido-methylcoumarins and was not inhibited by bestatin. The enzyme was inhibited by phenylmethylsulphonyl-fluoride, 3,4-dichloroisocoumarin, N-hydroxymercuriphenyl-sulphonic acid and N-ethylmaleimide showing that its activity is serine and cysteine dependent. The purified enzyme released tripeptides from several naturally occurring neuropeptides with quite broad specificity. Cholecystokinin octapeptide, angiotensin III and neurokinin A were the most rapidly hydrolysed. Peptides with Pro residues around the point of cleavage were not hydrolysed. | lld:pubmed |
| pubmed-article:8396212 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8396212 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8396212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8396212 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8396212 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8396212 | pubmed:issn | 0364-3190 | lld:pubmed |
| pubmed-article:8396212 | pubmed:author | pubmed-author:WilsonCC | lld:pubmed |
| pubmed-article:8396212 | pubmed:author | pubmed-author:McDermottJ... | lld:pubmed |
| pubmed-article:8396212 | pubmed:author | pubmed-author:GibsonA MAM | lld:pubmed |
| pubmed-article:8396212 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8396212 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:8396212 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8396212 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8396212 | pubmed:pagination | 743-9 | lld:pubmed |
| pubmed-article:8396212 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:8396212 | pubmed:meshHeading | pubmed-meshheading:8396212-... | lld:pubmed |
| pubmed-article:8396212 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8396212 | pubmed:articleTitle | Purification and characterization of tripeptidylpeptidase-II from post-mortem human brain. | lld:pubmed |
| pubmed-article:8396212 | pubmed:affiliation | MRC Neurochemical Pathology Unit, Newcastle General Hospital, United Kingdom. | lld:pubmed |
| pubmed-article:8396212 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:7174 | entrezgene:pubmed | pubmed-article:8396212 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8396212 | lld:pubmed |
