| pubmed-article:8391827 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C0206558 | lld:lifeskim |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C0684249 | lld:lifeskim |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C0237865 | lld:lifeskim |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C1336633 | lld:lifeskim |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C1517499 | lld:lifeskim |
| pubmed-article:8391827 | lifeskim:mentions | umls-concept:C1514926 | lld:lifeskim |
| pubmed-article:8391827 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:8391827 | pubmed:dateCreated | 1993-8-6 | lld:pubmed |
| pubmed-article:8391827 | pubmed:abstractText | One of the recent strategies for gene therapy as a cancer control is the targeted introduction of a drug-sensitivity gene into tumor cells. We investigated the gene transfer of herpes simplex virus type I thymidine kinase (HSV-TK) gene as a drug-sensitivity gene into human lung cancer cell lines. We used a recombinant retroviral vector derived from Moloney murine leukemia virus (MuLV) as one of potential vectors for gene therapy. The amphotropic retroviral vector consisted of the HSV-TK gene and the neomycin-resistant gene under Rous sarcoma virus (RSV) promoter control. The antiherpes drugs, acyclovir (ACV) and ganciclovir (GCV), were chosen for testing the activity of HSV-TK that was transferred into human lung cancer cell lines. ACV and GCV are nucleoside analogs specifically converted by HSV-TK to a toxic form capable of inhibiting DNA synthesis. The cytotoxicity was determined by using a tetrazolium-based colorimetric assay (MTT assay). The results obtained from our experiments demonstrated that the retroviral vector-mediated HSV-TK gene transfer leads to ACV- and GCV-dependent cytotoxicity in human lung cancer cell lines, which were both small cell carcinoma and non-small cell carcinoma established from human specimens. These findings suggest that the gene transfer of HSV-TK gene into tumor cells would be one of the models for the use of gene therapy to control lung cancer. | lld:pubmed |
| pubmed-article:8391827 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8391827 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8391827 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8391827 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8391827 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8391827 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:8391827 | pubmed:issn | 1044-1549 | lld:pubmed |
| pubmed-article:8391827 | pubmed:author | pubmed-author:HasegawaYY | lld:pubmed |
| pubmed-article:8391827 | pubmed:author | pubmed-author:SaitoHH | lld:pubmed |
| pubmed-article:8391827 | pubmed:author | pubmed-author:AbeAA | lld:pubmed |
| pubmed-article:8391827 | pubmed:author | pubmed-author:HasegawaTT | lld:pubmed |
| pubmed-article:8391827 | pubmed:author | pubmed-author:KawabeTT | lld:pubmed |
| pubmed-article:8391827 | pubmed:author | pubmed-author:ShimokataKK | lld:pubmed |
| pubmed-article:8391827 | pubmed:author | pubmed-author:FONJJ | lld:pubmed |
| pubmed-article:8391827 | pubmed:author | pubmed-author:KiriokaTT | lld:pubmed |
| pubmed-article:8391827 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8391827 | pubmed:volume | 8 | lld:pubmed |
| pubmed-article:8391827 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8391827 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8391827 | pubmed:pagination | 655-61 | lld:pubmed |
| pubmed-article:8391827 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:meshHeading | pubmed-meshheading:8391827-... | lld:pubmed |
| pubmed-article:8391827 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8391827 | pubmed:articleTitle | Gene transfer of herpes simplex virus type I thymidine kinase gene as a drug sensitivity gene into human lung cancer cell lines using retroviral vectors. | lld:pubmed |
| pubmed-article:8391827 | pubmed:affiliation | First Department of Internal Medicine, Nagoya University School of Medicine, Japan. | lld:pubmed |
| pubmed-article:8391827 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8391827 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8391827 | lld:pubmed |
