| pubmed-article:8382412 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8382412 | lifeskim:mentions | umls-concept:C0935640 | lld:lifeskim |
| pubmed-article:8382412 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:8382412 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8382412 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8382412 | lifeskim:mentions | umls-concept:C0872192 | lld:lifeskim |
| pubmed-article:8382412 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:8382412 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:8382412 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:8382412 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8382412 | pubmed:dateCreated | 1993-3-23 | lld:pubmed |
| pubmed-article:8382412 | pubmed:abstractText | Adult diarrheal rotavirus (ADRV) is a currently noncultivatable group B human rotavirus responsible for epidemic outbreaks of gastroenteritis in China. Gene segment 5 of ADRV encodes the major inner capsid protein, VP6. ADRV gene 5 was inserted into a recombinant baculovirus by homologous recombination between baculovirus shuttle plasmid pACYM1-AD5 and AcNPV genomic DNA. Baculovirus recombinants were selected visually and plaque purified and VP6 expression was detected by Coomassie staining of PAGE-separated proteins. The baculovirus-expressed gene 5 polypeptide is 44 kDa, the same as for the major inner capsid protein present on EDTA-treated ADRV virions and in vitro-expressed VP6 protein. The expressed protein is oligomeric and in the absence of reducing agents multimerizes to apparent trimer, hexamer, and greater molecular mass as assayed by SDS-PAGE. The VP6 protein is immunoprecipitable by hyperimmune serum to ADRV, human ADRV convalescent serum, by a group B-specific monoclonal antibody and by porcine group B rotavirus infection serum. The baculovirus-expressed protein is immunogenic and antibodies to the expressed protein recognize ADRV virions. The ADRV VP6 protein should be useful for developing diagnostic assays for serum antibodies to group B rotavirus as well as for generating hyperimmune serum and monoclonal antibodies for detecting viral antigen from ADRV and other group B rotaviruses. | lld:pubmed |
| pubmed-article:8382412 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382412 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382412 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8382412 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382412 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8382412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382412 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382412 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8382412 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:8382412 | pubmed:issn | 0042-6822 | lld:pubmed |
| pubmed-article:8382412 | pubmed:author | pubmed-author:HungTT | lld:pubmed |
| pubmed-article:8382412 | pubmed:author | pubmed-author:JanI RIR | lld:pubmed |
| pubmed-article:8382412 | pubmed:author | pubmed-author:ChenGG | lld:pubmed |
| pubmed-article:8382412 | pubmed:author | pubmed-author:WangZ JZJ | lld:pubmed |
| pubmed-article:8382412 | pubmed:author | pubmed-author:MackowE RER | lld:pubmed |
| pubmed-article:8382412 | pubmed:author | pubmed-author:Werner-Eckert... | lld:pubmed |
| pubmed-article:8382412 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8382412 | pubmed:volume | 193 | lld:pubmed |
| pubmed-article:8382412 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8382412 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8382412 | pubmed:pagination | 537-42 | lld:pubmed |
| pubmed-article:8382412 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8382412 | pubmed:meshHeading | pubmed-meshheading:8382412-... | lld:pubmed |
| pubmed-article:8382412 | pubmed:meshHeading | pubmed-meshheading:8382412-... | lld:pubmed |
| pubmed-article:8382412 | pubmed:meshHeading | pubmed-meshheading:8382412-... | lld:pubmed |
| pubmed-article:8382412 | pubmed:meshHeading | pubmed-meshheading:8382412-... | lld:pubmed |
| pubmed-article:8382412 | pubmed:meshHeading | pubmed-meshheading:8382412-... | lld:pubmed |
| pubmed-article:8382412 | pubmed:meshHeading | pubmed-meshheading:8382412-... | lld:pubmed |
| pubmed-article:8382412 | pubmed:meshHeading | pubmed-meshheading:8382412-... | lld:pubmed |
| pubmed-article:8382412 | pubmed:meshHeading | pubmed-meshheading:8382412-... | lld:pubmed |
| pubmed-article:8382412 | pubmed:meshHeading | pubmed-meshheading:8382412-... | lld:pubmed |
| pubmed-article:8382412 | pubmed:meshHeading | pubmed-meshheading:8382412-... | lld:pubmed |
| pubmed-article:8382412 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8382412 | pubmed:articleTitle | Baculovirus expression of the ADRV gene 5 encoded protein produces an oligomerized, antigenic, and immunogenic VP6 protein. | lld:pubmed |
| pubmed-article:8382412 | pubmed:affiliation | Department of Veterans Affairs, Northport VA Medical Center, New York 11733. | lld:pubmed |
| pubmed-article:8382412 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8382412 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8382412 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8382412 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
