| pubmed-article:8382267 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8382267 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8382267 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:8382267 | lifeskim:mentions | umls-concept:C1823153 | lld:lifeskim |
| pubmed-article:8382267 | lifeskim:mentions | umls-concept:C2349976 | lld:lifeskim |
| pubmed-article:8382267 | lifeskim:mentions | umls-concept:C1552644 | lld:lifeskim |
| pubmed-article:8382267 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:8382267 | lifeskim:mentions | umls-concept:C0205923 | lld:lifeskim |
| pubmed-article:8382267 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8382267 | pubmed:dateCreated | 1993-3-24 | lld:pubmed |
| pubmed-article:8382267 | pubmed:abstractText | A gamma-aminobutyric acidA (GABAA) receptor (GABAAR) gamma 2 subunit (short form) was cloned from an adult human cerebral cortex cDNA library in bacteriophage lambda gt11. The 261-bp intracellular loop (IL) located between M3 and M4 was amplified using the polymerase chain reaction and inserted into the expression vectors lambda gt11 and pGEX-3X. Both beta-galactosidase (LacZ) and glutathione-S-transferase (GST) fusion proteins containing the gamma 2IL were purified, and a rabbit antibody to the LacZ-gamma 2IL was made. The antibody reacted with the gamma 2IL of both LacZ and GST fusion proteins and immunoprecipitated the GABAAR/benzodiazepine receptor (GABAAR/BZDR) from bovine and rat brain. The antibody reacted in affinity-purified GABAAR/BZDR immunoblots with a wide peptide band of 44,000-49,000 M(r). Immunoprecipitation studies with the anti-gamma 2IL antibody suggest that in the cerebral cortex, 87% of the GABAARs with high affinity for benzodiazepines and 70% of the GABAARs with high affinity for muscimol contain at least a gamma subunit, probably a gamma 2. These results indicate that there are [3H]muscimol binding GABAARs that do not bind [3H]flunitrazepam with high affinity. Immunoprecipitations with this and other anti-GABAAR/BZDR antibodies indicate that the most abundant combination of GABAAR subunits in the cerebral cortex involves alpha 1, gamma 2 (or other gamma), and beta 2 and/or beta 3 subunits. These subunits coexist in > 60% of the GABAAR/BZDRs in the cerebral cortex. The results also show that a considerable proportion (20-25%) of the cerebellar GABAAR/BZDRs is clonazepam insensitive. At least 74% of these cerebellar receptors, which likely contain alpha 6, also contain gamma 2 (or other gamma) subunit(s). The alpha 1 and beta 2 or beta 3 subunits are also frequently associated with gamma 2 (or other gamma) and alpha 6 in these cerebellar receptors. | lld:pubmed |
| pubmed-article:8382267 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382267 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8382267 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382267 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8382267 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382267 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382267 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382267 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8382267 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8382267 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:8382267 | pubmed:issn | 0022-3042 | lld:pubmed |
| pubmed-article:8382267 | pubmed:author | pubmed-author:FernandoL PLP | lld:pubmed |
| pubmed-article:8382267 | pubmed:author | pubmed-author:MalletJJ | lld:pubmed |
| pubmed-article:8382267 | pubmed:author | pubmed-author:KhanZ UZU | lld:pubmed |
| pubmed-article:8382267 | pubmed:author | pubmed-author:De BlasA LAL | lld:pubmed |
| pubmed-article:8382267 | pubmed:author | pubmed-author:BusquetsXX | lld:pubmed |
| pubmed-article:8382267 | pubmed:author | pubmed-author:MirallesC PCP | lld:pubmed |
| pubmed-article:8382267 | pubmed:author | pubmed-author:EscribáPP | lld:pubmed |
| pubmed-article:8382267 | pubmed:author | pubmed-author:FillaMM | lld:pubmed |
| pubmed-article:8382267 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8382267 | pubmed:volume | 60 | lld:pubmed |
| pubmed-article:8382267 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8382267 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8382267 | pubmed:pagination | 961-71 | lld:pubmed |
| pubmed-article:8382267 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8382267 | pubmed:meshHeading | pubmed-meshheading:8382267-... | lld:pubmed |
| pubmed-article:8382267 | pubmed:meshHeading | pubmed-meshheading:8382267-... | lld:pubmed |
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| pubmed-article:8382267 | pubmed:meshHeading | pubmed-meshheading:8382267-... | lld:pubmed |
| pubmed-article:8382267 | pubmed:meshHeading | pubmed-meshheading:8382267-... | lld:pubmed |
| pubmed-article:8382267 | pubmed:meshHeading | pubmed-meshheading:8382267-... | lld:pubmed |
| pubmed-article:8382267 | pubmed:meshHeading | pubmed-meshheading:8382267-... | lld:pubmed |
| pubmed-article:8382267 | pubmed:meshHeading | pubmed-meshheading:8382267-... | lld:pubmed |
| pubmed-article:8382267 | pubmed:meshHeading | pubmed-meshheading:8382267-... | lld:pubmed |
| pubmed-article:8382267 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8382267 | pubmed:articleTitle | Antibodies to the human gamma 2 subunit of the gamma-aminobutyric acidA/benzodiazepine receptor. | lld:pubmed |
| pubmed-article:8382267 | pubmed:affiliation | Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri, Kansas City 64110-2499. | lld:pubmed |
| pubmed-article:8382267 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8382267 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8382267 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8382267 | lld:pubmed |
