| pubmed-article:8381456 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8381456 | lifeskim:mentions | umls-concept:C1708248 | lld:lifeskim |
| pubmed-article:8381456 | lifeskim:mentions | umls-concept:C0032005 | lld:lifeskim |
| pubmed-article:8381456 | lifeskim:mentions | umls-concept:C0019929 | lld:lifeskim |
| pubmed-article:8381456 | lifeskim:mentions | umls-concept:C2003941 | lld:lifeskim |
| pubmed-article:8381456 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:8381456 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8381456 | pubmed:dateCreated | 1993-3-8 | lld:pubmed |
| pubmed-article:8381456 | pubmed:abstractText | The present study has characterized the gonadotrophin-releasing hormone (GnRH) receptor in immortalized alpha T3-1 pituitary gonadotroph cells. GnRH and GnRH analogues produced both a dose- and time-dependent increase in total inositol phosphate (IP) accumulation. The rank order of potency of these analogues was the same as that obtained in parallel receptor-binding studies in alpha T3-1 cells. These responses were abolished following pretreatment with a GnRH antagonist. The use of a specific inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) assay demonstrated a rapid but short-lived rise in Ins(1,4,5)P3 production. Intracellular calcium ([Ca2+]i) was subsequently measured in alpha T3-1 cells using dual wavelength fluorescence microscopy combined with dynamic video imaging. GnRH produced a biphasic rise in [Ca2+]i. The initial calcium transient was complete within seconds while the smaller secondary plateau phase lasted several minutes. G-protein involvement in the IP response to GnRH in alpha T3-1 cells was investigated using sodium fluoride (NaF) and pertussis toxin (PTx) which activate and inactivate G-proteins respectively. Like GnRH, NaF produced a dose- and time-dependent increase in IP accumulation. Activation of phospholipase C in these cells by either GnRH or NaF was PTx-insensitive, suggesting that the G-protein involved was neither Gi nor Go but more probably Gq. Immunoblot analysis of alpha T3-1 cell membranes using antisera raised against the predicted C-terminal decapeptide of the alpha subunit of Gq demonstrated the presence of Gq in alpha T3-1 cells. Collectively these results show that the GnRH receptors expressed in alpha T3-1 cells are coupled to the phosphatidylinositol second messenger pathway via a specific G-protein.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:8381456 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8381456 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381456 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8381456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381456 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381456 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8381456 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8381456 | pubmed:issn | 0022-0795 | lld:pubmed |
| pubmed-article:8381456 | pubmed:author | pubmed-author:AndersonLL | lld:pubmed |
| pubmed-article:8381456 | pubmed:author | pubmed-author:MilliganGG | lld:pubmed |
| pubmed-article:8381456 | pubmed:author | pubmed-author:EidneK AKA | lld:pubmed |
| pubmed-article:8381456 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8381456 | pubmed:volume | 136 | lld:pubmed |
| pubmed-article:8381456 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8381456 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8381456 | pubmed:pagination | 51-8 | lld:pubmed |
| pubmed-article:8381456 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8381456 | pubmed:meshHeading | pubmed-meshheading:8381456-... | lld:pubmed |
| pubmed-article:8381456 | pubmed:meshHeading | pubmed-meshheading:8381456-... | lld:pubmed |
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| pubmed-article:8381456 | pubmed:meshHeading | pubmed-meshheading:8381456-... | lld:pubmed |
| pubmed-article:8381456 | pubmed:meshHeading | pubmed-meshheading:8381456-... | lld:pubmed |
| pubmed-article:8381456 | pubmed:meshHeading | pubmed-meshheading:8381456-... | lld:pubmed |
| pubmed-article:8381456 | pubmed:meshHeading | pubmed-meshheading:8381456-... | lld:pubmed |
| pubmed-article:8381456 | pubmed:meshHeading | pubmed-meshheading:8381456-... | lld:pubmed |
| pubmed-article:8381456 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8381456 | pubmed:articleTitle | Characterization of the gonadotrophin-releasing hormone receptor in alpha T3-1 pituitary gonadotroph cells. | lld:pubmed |
| pubmed-article:8381456 | pubmed:affiliation | MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, U.K. | lld:pubmed |
| pubmed-article:8381456 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8381456 | lld:pubmed |
