| pubmed-article:8381205 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8381205 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:8381205 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8381205 | lifeskim:mentions | umls-concept:C0084133 | lld:lifeskim |
| pubmed-article:8381205 | lifeskim:mentions | umls-concept:C0041221 | lld:lifeskim |
| pubmed-article:8381205 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:8381205 | lifeskim:mentions | umls-concept:C1332838 | lld:lifeskim |
| pubmed-article:8381205 | lifeskim:mentions | umls-concept:C0332453 | lld:lifeskim |
| pubmed-article:8381205 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8381205 | pubmed:dateCreated | 1993-3-3 | lld:pubmed |
| pubmed-article:8381205 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:abstractText | Analysis of gene expression in Trypanosoma cruzi has been impeded by the lack of efficient, stable, DNA-mediated transfection systems. We describe here the establishment of such a system for T. cruzi. Stable transformants were isolated following integration of the circular transforming plasmid into the chromosome by homologous recombination. Mutants with a disrupted PUB12.5 polyubiquitin gene, resulting from targeted integration of the plasmid vector, have been isolated. A mutant harboring the disrupted PUB12.5 gene lacks the intact PUB12.5 mRNA as well as transcripts corresponding to the truncated gene. Genomic Southern-blot analysis indicates that the inserted plasmid is tandemly repeated in each of the clones analyzed. A secondary recombination event in one clone resulted in a deletion within the 2.65 calmodulin-ubiquitin locus, encompassing the sequence from the CalA2 calmodulin gene to the PUB12.5 polyubiquitin gene. | lld:pubmed |
| pubmed-article:8381205 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8381205 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8381205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8381205 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8381205 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8381205 | pubmed:issn | 0166-6851 | lld:pubmed |
| pubmed-article:8381205 | pubmed:author | pubmed-author:HariharanSS | lld:pubmed |
| pubmed-article:8381205 | pubmed:author | pubmed-author:SwindleJJ | lld:pubmed |
| pubmed-article:8381205 | pubmed:author | pubmed-author:AjiokaJJ | lld:pubmed |
| pubmed-article:8381205 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8381205 | pubmed:volume | 57 | lld:pubmed |
| pubmed-article:8381205 | pubmed:geneSymbol | PUB12.5 | lld:pubmed |
| pubmed-article:8381205 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8381205 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8381205 | pubmed:pagination | 15-30 | lld:pubmed |
| pubmed-article:8381205 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8381205 | pubmed:meshHeading | pubmed-meshheading:8381205-... | lld:pubmed |
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| pubmed-article:8381205 | pubmed:meshHeading | pubmed-meshheading:8381205-... | lld:pubmed |
| pubmed-article:8381205 | pubmed:meshHeading | pubmed-meshheading:8381205-... | lld:pubmed |
| pubmed-article:8381205 | pubmed:meshHeading | pubmed-meshheading:8381205-... | lld:pubmed |
| pubmed-article:8381205 | pubmed:meshHeading | pubmed-meshheading:8381205-... | lld:pubmed |
| pubmed-article:8381205 | pubmed:meshHeading | pubmed-meshheading:8381205-... | lld:pubmed |
| pubmed-article:8381205 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8381205 | pubmed:articleTitle | Stable transformation of Trypanosoma cruzi: inactivation of the PUB12.5 polyubiquitin gene by targeted gene disruption. | lld:pubmed |
| pubmed-article:8381205 | pubmed:affiliation | Department of Microbiology and Immunology, University of Tennessee, Memphis 38163. | lld:pubmed |
| pubmed-article:8381205 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8381205 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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