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pubmed-article:8373811pubmed:abstractTextA cDNA encoding bovine procathepsin B was isolated. The deduced amino acid sequence revealed that a stop (TAG) codon, instead of a Trp-257 codon (TGG), generates in bovine a cathepsin B precursor four amino acids shorter than in other species. Because micro-heterogeneities were previously reported in the cathepsin B primary structure, sequence polymorphism in the protein coding region was then investigated by PCR sequencing of genomic fragments and RNase protection assays. Experiments performed with 12-15 animals of three breeds did not reveal any difference with our cDNA sequence. We conclude that sequence polymorphism in bovine cathepsin B is a rare event, and can only result from the expression of different alleles of a unique gene.lld:pubmed
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pubmed-article:8373811pubmed:articleTitleNucleotide sequence of bovine preprocathepsin B. A study of polymorphism in the protein coding region.lld:pubmed
pubmed-article:8373811pubmed:affiliationUnité de Recherches sur l'Expression des Protéases, Institut National de la Recherche Agronomique, Ceyrat, France.lld:pubmed
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