| pubmed-article:8363605 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8363605 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:8363605 | lifeskim:mentions | umls-concept:C0242692 | lld:lifeskim |
| pubmed-article:8363605 | lifeskim:mentions | umls-concept:C0049700 | lld:lifeskim |
| pubmed-article:8363605 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
| pubmed-article:8363605 | lifeskim:mentions | umls-concept:C0175921 | lld:lifeskim |
| pubmed-article:8363605 | lifeskim:mentions | umls-concept:C0178499 | lld:lifeskim |
| pubmed-article:8363605 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:8363605 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8363605 | pubmed:dateCreated | 1993-9-28 | lld:pubmed |
| pubmed-article:8363605 | pubmed:abstractText | The role of Cys-138 in the catalysis of the skeletal muscle 6-phosphofructo-2-kinase reaction was investigated by mutating this residue to serine, glutamine and alanine, expressing the mutants in E. coli with a T7 RNA polymerase-based expression system, and analyzing their kinetic properties. The Cys138Ala mutant had greatly diminished activity, while the Cys138Ser and Cys138Gln mutants had maximal velocities 2-3 fold higher than the wild-type enzyme. It was concluded that Cys-138 does not act as a base catalyst in the kinase reaction, but that it plays a significant structural role in the enzyme's active site. | lld:pubmed |
| pubmed-article:8363605 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8363605 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8363605 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8363605 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8363605 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8363605 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8363605 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8363605 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8363605 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8363605 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8363605 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:8363605 | pubmed:author | pubmed-author:PilkisS JSJ | lld:pubmed |
| pubmed-article:8363605 | pubmed:author | pubmed-author:KurlandI JIJ | lld:pubmed |
| pubmed-article:8363605 | pubmed:author | pubmed-author:el-MaghrabiM... | lld:pubmed |
| pubmed-article:8363605 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8363605 | pubmed:day | 31 | lld:pubmed |
| pubmed-article:8363605 | pubmed:volume | 195 | lld:pubmed |
| pubmed-article:8363605 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8363605 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8363605 | pubmed:pagination | 229-36 | lld:pubmed |
| pubmed-article:8363605 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:meshHeading | pubmed-meshheading:8363605-... | lld:pubmed |
| pubmed-article:8363605 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8363605 | pubmed:articleTitle | Lack of evidence for a role of Cys-138 as a base catalyst in the skeletal muscle 6-phosphofructo-2-kinase reaction. | lld:pubmed |
| pubmed-article:8363605 | pubmed:affiliation | Department of Physiology and Biophysics, State University of New York, Stony Brook 11794-8661. | lld:pubmed |
| pubmed-article:8363605 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8363605 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8363605 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8363605 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
