| pubmed-article:8357941 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8357941 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:8357941 | lifeskim:mentions | umls-concept:C1524119 | lld:lifeskim |
| pubmed-article:8357941 | lifeskim:mentions | umls-concept:C0268532 | lld:lifeskim |
| pubmed-article:8357941 | lifeskim:mentions | umls-concept:C0872318 | lld:lifeskim |
| pubmed-article:8357941 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:8357941 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:8357941 | pubmed:dateCreated | 1993-9-29 | lld:pubmed |
| pubmed-article:8357941 | pubmed:abstractText | It has been reported that the urine of patients with prolidase deficiency contains various iminodipeptides with a carboxyl-terminal proline (hydroxyproline). These iminodipeptides have hitherto been detected indirectly by acid hydrolysis or enzymatic digestion, followed by amino acid analysis. In the present study, it was shown that X-Pro could be distinguished from Pro-X when the iminodipeptides were analysed directly by liquid chromatography coupled with atmospheric pressure ionization mass spectrometry (LC/API-MS), with scanning of the protonated molecule ions ([M+H]+). The same procedure also successfully quantified urinary iminodipeptides from patients with prolidase deficiency. A quantitative investigation of two siblings with prolidase deficiency revealed that the patient with severe clinical symptoms excreted more iminodipeptides than the other who did not have serious symptoms. LC/API-MS also revealed iminodipeptides (Gly-Hyp and Pro-Hyp) in the urine of the mother of the patients and in normal volunteers. Patients excreted much more Pro-Hyp than normal volunteers, whereas no quantitative differences were found between the mother and controls. In patients, the excretion of large quantities of X-Pro is due to their very low prolidase activity towards this type of substrate. In the erythrocytes of patients, prolidase activity towards X-Hyp was extremely low; even in the mother and normal volunteers, it was remarkably low in comparison with the activity against X-Pro. | lld:pubmed |
| pubmed-article:8357941 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8357941 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8357941 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8357941 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8357941 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8357941 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8357941 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8357941 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8357941 | pubmed:month | May | lld:pubmed |
| pubmed-article:8357941 | pubmed:issn | 0939-4974 | lld:pubmed |
| pubmed-article:8357941 | pubmed:author | pubmed-author:OhnoTT | lld:pubmed |
| pubmed-article:8357941 | pubmed:author | pubmed-author:KodamaHH | lld:pubmed |
| pubmed-article:8357941 | pubmed:author | pubmed-author:SugaharaKK | lld:pubmed |
| pubmed-article:8357941 | pubmed:author | pubmed-author:ArataJJ | lld:pubmed |
| pubmed-article:8357941 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8357941 | pubmed:volume | 31 | lld:pubmed |
| pubmed-article:8357941 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8357941 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8357941 | pubmed:pagination | 317-22 | lld:pubmed |
| pubmed-article:8357941 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:meshHeading | pubmed-meshheading:8357941-... | lld:pubmed |
| pubmed-article:8357941 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8357941 | pubmed:articleTitle | The use of liquid chromatography-mass spectrometry for the identification and quantification of urinary iminodipeptides in prolidase deficiency. | lld:pubmed |
| pubmed-article:8357941 | pubmed:affiliation | Department of Chemistry, Kochi Medical School, Japan. | lld:pubmed |
| pubmed-article:8357941 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8357941 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
