| pubmed-article:8350864 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8350864 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:8350864 | lifeskim:mentions | umls-concept:C0596290 | lld:lifeskim |
| pubmed-article:8350864 | lifeskim:mentions | umls-concept:C0597295 | lld:lifeskim |
| pubmed-article:8350864 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:8350864 | lifeskim:mentions | umls-concept:C0851827 | lld:lifeskim |
| pubmed-article:8350864 | lifeskim:mentions | umls-concept:C1701901 | lld:lifeskim |
| pubmed-article:8350864 | lifeskim:mentions | umls-concept:C0286382 | lld:lifeskim |
| pubmed-article:8350864 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8350864 | pubmed:dateCreated | 1993-9-10 | lld:pubmed |
| pubmed-article:8350864 | pubmed:abstractText | The effect of beta-alanyl-L-histidinato zinc (AHZ) on bone metabolism was investigated in osteoblastic MC3T3-E1 cells. Cells were cultured for 3 days at 37 degrees C in a CO2 incubator in plastic dishes containing alpha-modified minimum essential medium supplemented with 10% fetal bovine serum. After the cultures, the medium was exchanged for that containing 0.1% bovine serum albumin plus various concentrations of AHZ or other reagents, and the cells were cultured further for appropriate periods of time. The presence of AHZ (10(-7)-10(-5) M) stimulated the proliferation of cells. AHZ (10(-6) and 10(-5) M) increased deoxyribonucleic acid (DNA) content in the cells with 48 hr-culture. This increase was completely blocked by the presence of cycloheximide (10(-6) M) or hydroxyurea (10(-3) M). Also, the presence of cycloheximide (10(-6) M) completely inhibited the AHZ (10(-5) M)-induced increase in the proliferation of cells. Meanwhile, parathyroid hormone (10(-7) M), estrogen (10(-9) M) and insulin (10(-8) M) significantly increased cellular DNA content. However, these hormonal effects clearly lowered in comparison with that of AHZ (10(-5) M). Dibutyryl cyclic AMP (10(-4) M) and zinc sulfate (10(-5) M) did not cause a significant increase in cellular DNA content. The present results support the view that AHZ has a direct specific proliferative effect on osteoblastic cells in vitro and that this effect is dependent on protein synthesis. | lld:pubmed |
| pubmed-article:8350864 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8350864 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8350864 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8350864 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8350864 | pubmed:month | May | lld:pubmed |
| pubmed-article:8350864 | pubmed:issn | 0300-8177 | lld:pubmed |
| pubmed-article:8350864 | pubmed:author | pubmed-author:YamaguchiMM | lld:pubmed |
| pubmed-article:8350864 | pubmed:author | pubmed-author:HashizumeMM | lld:pubmed |
| pubmed-article:8350864 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8350864 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:8350864 | pubmed:volume | 122 | lld:pubmed |
| pubmed-article:8350864 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8350864 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8350864 | pubmed:pagination | 59-64 | lld:pubmed |
| pubmed-article:8350864 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:8350864 | pubmed:meshHeading | pubmed-meshheading:8350864-... | lld:pubmed |
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| pubmed-article:8350864 | pubmed:meshHeading | pubmed-meshheading:8350864-... | lld:pubmed |
| pubmed-article:8350864 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8350864 | pubmed:articleTitle | Stimulatory effect of beta-alanyl-L-histidinato zinc on cell proliferation is dependent on protein synthesis in osteoblastic MC3T3-E1 cells. | lld:pubmed |
| pubmed-article:8350864 | pubmed:affiliation | Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, Japan. | lld:pubmed |
| pubmed-article:8350864 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8350864 | lld:pubmed |
