Clinical impact of breakpoint position within M-bcr in chronic myeloid leukemia.

Source:http://linkedlifedata.com/resource/pubmed/id/8350622

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Authors

Mitelman F, Turesson I, Nilsson PG, Aman P, Simonsson B, Hein P, Mahé C, Kristoffersson U, Fioretos T

Affiliation

Department of Clinical Genetics, Lund University Hospital, Sweden.

Abstract

We have analyzed the M-bcr breakpoint position in 133 Philadelphia-positive chronic myeloid leukemia patients and correlated the findings with clinical, hematologic, and cytogenetic data. We also investigated the splicing pattern of the BCR-ABL mRNA in 30 patients, using reverse transcriptase PCR. No statistically significant differences were found between breakpoint position within M-bcr and clinical parameters at diagnosis, the karyotypic evolution pattern, or the leukemic phenotype during blast crisis. Furthermore, the breakpoint position within M-bcr did not correlate with the duration of chronic phase or survival time. When the splicing pattern of the BCR-ABL mRNA was compared with the results of the genomic breakpoint mapping, it was found that approximately 60% (8/14) of the patients with a 5' break expressed b2a2 fusion mRNA, whereas all patients (10/10) with a 3' break expressed b3a2 BCR-ABL mRNA.

PMID
8350622

Publication types

Research Support, Non-U.S. Gov't