| pubmed-article:8310898 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8310898 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8310898 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:8310898 | lifeskim:mentions | umls-concept:C1123019 | lld:lifeskim |
| pubmed-article:8310898 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:8310898 | lifeskim:mentions | umls-concept:C0023607 | lld:lifeskim |
| pubmed-article:8310898 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:8310898 | lifeskim:mentions | umls-concept:C0034580 | lld:lifeskim |
| pubmed-article:8310898 | lifeskim:mentions | umls-concept:C1522485 | lld:lifeskim |
| pubmed-article:8310898 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8310898 | pubmed:dateCreated | 1994-3-14 | lld:pubmed |
| pubmed-article:8310898 | pubmed:abstractText | A radioimmunoassay for bovine (bLH), ovine (oLH) and porcine (pLH) luteinizing hormone was developed using a human 125ILH tracer from a commercial kit and a monoclonal antibody (518B7) specific for LH but with low species specificity. Standard curves demonstrated similar binding kinetics when bLH, oLH and pLH were incubated with tracer and antibody for 2 h at room temperature. A 30-min delay in the addition of the tracer gave sufficient sensitivity when analysing pLH. Separation of antibody-bound LH from free hormone was achieved by using second antibody-coated micro Sepharose beads. The assay was validated and the performance compared with that of an RIA currently in use for determination of bLH and oLH (coefficient of correlation: 0.99 and 0.98). Regardless of the standards used, intra-assay coefficients of variation were < 10% for LH concentrations exceeding 1 microgram/L. The inter-assay coefficients of variation were < 15%. The assay was used for clinical evaluation demonstrating the pre-ovulatory LH surge in two cyclic cows, LH pulsatility in an oophorectomized ewe and LH response to GnRH injection in a boar. | lld:pubmed |
| pubmed-article:8310898 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8310898 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8310898 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8310898 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8310898 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8310898 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8310898 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8310898 | pubmed:issn | 0044-605X | lld:pubmed |
| pubmed-article:8310898 | pubmed:author | pubmed-author:MadejAA | lld:pubmed |
| pubmed-article:8310898 | pubmed:author | pubmed-author:MolinaJ RJR | lld:pubmed |
| pubmed-article:8310898 | pubmed:author | pubmed-author:TagleRR | lld:pubmed |
| pubmed-article:8310898 | pubmed:author | pubmed-author:ForsbergMM | lld:pubmed |
| pubmed-article:8310898 | pubmed:author | pubmed-author:CarlssonM AMA | lld:pubmed |
| pubmed-article:8310898 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8310898 | pubmed:volume | 34 | lld:pubmed |
| pubmed-article:8310898 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8310898 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8310898 | pubmed:pagination | 255-62 | lld:pubmed |
| pubmed-article:8310898 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:meshHeading | pubmed-meshheading:8310898-... | lld:pubmed |
| pubmed-article:8310898 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8310898 | pubmed:articleTitle | Radioimmunoassay of bovine, ovine and porcine luteinizing hormone with a monoclonal antibody and a human tracer. | lld:pubmed |
| pubmed-article:8310898 | pubmed:affiliation | Department of Clinical Chemistry, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala. | lld:pubmed |
| pubmed-article:8310898 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8310898 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8310898 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8310898 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8310898 | lld:pubmed |
