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pubmed-article:8305460pubmed:abstractTextThe present study was aimed at investigating the spontaneous transfer of fluorescently labelled serine- and choline-glycerophospholipids from unilamellar vesicles to resting human blood platelets. The most effectively transferred phospholipids were pyrene-phosphatidylserine (PS) and the ether analogues of choline-glycerophospholipids, e.g., pyrene-alkylacyl- and pyrene-1'-alkenylacyl-glycerophosphocholines (plasmalogens). Transfer of pyrene-diacyl-glycerophosphocholine and pyrene-phosphatidic acid was almost not detectable under the same experimental conditions. The fast intermembrane PS-transfer could be explained by the very high degree of adsorption of PS donor vesicles to the platelet plasma membrane. The short halftime of transfer rate (12-14 min) and the high incorporation (1.08-2.16% of total platelet glycerophosphocholines) observed for ether choline-phospholipids in contrast to pyrene-PS (20 min, 0.8% of total platelet PS), could be interpreted in terms of their bulk membrane properties.lld:pubmed
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pubmed-article:8305460pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:8305460pubmed:year1994lld:pubmed
pubmed-article:8305460pubmed:articleTitleTransfer of pyrene-labelled diacyl-, alkylacyl-, and alkenylacyl-glycerophospholipids from vesicles to human blood platelets.lld:pubmed
pubmed-article:8305460pubmed:affiliationPhilipps Universität, Zentrum Innere Medizin, Abteilung Endokrinologie und Stoffwechsel, Marburg/Lahn, Germany.lld:pubmed
pubmed-article:8305460pubmed:publicationTypeJournal Articlelld:pubmed
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pubmed-article:8305460pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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