| pubmed-article:8302824 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8302824 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:8302824 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:8302824 | lifeskim:mentions | umls-concept:C0312586 | lld:lifeskim |
| pubmed-article:8302824 | lifeskim:mentions | umls-concept:C1704222 | lld:lifeskim |
| pubmed-article:8302824 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8302824 | pubmed:dateCreated | 1994-3-8 | lld:pubmed |
| pubmed-article:8302824 | pubmed:abstractText | The human MAGE-1 gene encodes a melanoma peptide antigen recognized by autologous cytotoxic T lymphocytes. To produce antibodies against the MAGE-1 gene product, several approaches were taken. Three oligopeptides were synthesized based on predicted MAGE-1 amino acid sequences and were used to generate rabbit anti-peptide anti-sera. In addition, a truncated MAGE-1 cDNA was cloned into an Escherichia coli expression vector, and recombinant protein was produced and purified. All three rabbit anti-peptide antisera showed reactivity against the immunizing peptide, and one reacted with the recombinant MAGE-1 protein by immunoblotting, but none reacted with cell lysates from MAGE-1 mRNA-positive cells. The recombinant MAGE-1 protein was then used for the generation of mouse monoclonal and rabbit polyclonal antibodies. One IgG1 monoclonal antibody, MA454, as well as rabbit polyclonal antisera recognized a 46-kDa protein in extracts of MAGE-1 mRNA-positive melanoma cell lines. The antibodies showed no apparent cross-reactivity with products of the closely related MAGE-2 and MAGE-3 genes. Serological typing of normal and tumor cell lysates was in full agreement with mRNA analysis, showing expression of MAGE-1 protein in MAGE-1 mRNA-positive testis and a subset of melanomas but not in MAGE-1 mRNA-negative normal or tumor tissues. Transfection of the MAGE-1 gene into a MAGE-1 mRNA-negative melanoma cell line resulted in the expression of the 46-kDa protein, confirming the identity of this protein as the MAGE-1 gene product. | lld:pubmed |
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| pubmed-article:8302824 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8302824 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8302824 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8302824 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8302824 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8302824 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:8302824 | pubmed:author | pubmed-author:StockertEE | lld:pubmed |
| pubmed-article:8302824 | pubmed:author | pubmed-author:OldL JLJ | lld:pubmed |
| pubmed-article:8302824 | pubmed:author | pubmed-author:ChenYY | lld:pubmed |
| pubmed-article:8302824 | pubmed:author | pubmed-author:BoonTT | lld:pubmed |
| pubmed-article:8302824 | pubmed:author | pubmed-author:ChenY TYT | lld:pubmed |
| pubmed-article:8302824 | pubmed:author | pubmed-author:RettigW JWJ | lld:pubmed |
| pubmed-article:8302824 | pubmed:author | pubmed-author:Garin-ChesaPP | lld:pubmed |
| pubmed-article:8302824 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:8302824 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8302824 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8302824 | pubmed:volume | 91 | lld:pubmed |
| pubmed-article:8302824 | pubmed:geneSymbol | MAGE-3 | lld:pubmed |
| pubmed-article:8302824 | pubmed:geneSymbol | MAGE-1 | lld:pubmed |
| pubmed-article:8302824 | pubmed:geneSymbol | MAGE-2 | lld:pubmed |
| pubmed-article:8302824 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8302824 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8302824 | pubmed:pagination | 1004-8 | lld:pubmed |
| pubmed-article:8302824 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:8302824 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8302824 | pubmed:articleTitle | Identification of the MAGE-1 gene product by monoclonal and polyclonal antibodies. | lld:pubmed |
| pubmed-article:8302824 | pubmed:affiliation | Ludwig Institute for Cancer Research, New York Unit, NY. | lld:pubmed |
| pubmed-article:8302824 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:4100 | entrezgene:pubmed | pubmed-article:8302824 | lld:entrezgene |
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