| pubmed-article:8272535 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8272535 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:8272535 | lifeskim:mentions | umls-concept:C0037868 | lld:lifeskim |
| pubmed-article:8272535 | lifeskim:mentions | umls-concept:C1707391 | lld:lifeskim |
| pubmed-article:8272535 | lifeskim:mentions | umls-concept:C0439536 | lld:lifeskim |
| pubmed-article:8272535 | lifeskim:mentions | umls-concept:C1516240 | lld:lifeskim |
| pubmed-article:8272535 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8272535 | pubmed:dateCreated | 1994-2-1 | lld:pubmed |
| pubmed-article:8272535 | pubmed:abstractText | Hyaluronic acid (HA) was used to select spermatozoa from frozen-thawed semen from five bulls; two were highly fertile and three had low fertility related to low post-thaw motility of the spermatozoa. Spermatozoa were collected after 1 h of self-migration in one of two media: a modified Tyrode's lactate solution (TALP) or a medium consisting of equal volumes of TALP and phosphate-buffered saline to which HA was added to obtain a final concentration of 1 mg mL-1 (HA medium). The selected spermatozoa were used to inseminate oocytes matured in vitro at a concentration of either 1.0 x 10(6) or 0.5 x 10(6) mL-1, constituting a 5 x 2 x 2 factorial experiment. After 19-22 h of sperm-oocyte co-incubation, fertilization of oocytes was assessed. The two concentrations of spermatozoa differed significantly in the percentage of oocytes penetrated (P = 0.001) and the percentage of oocytes with two pronuclei (P = 0.002). When 1.0 x 10(6) mL-1 spermatozoa were used, spermatozoa collected after swim-up in the HA medium fertilized more oocytes (P = 0.01) than did those collected after swim-up in TALP. Variation between bulls was also significant (P = 0.002). The beneficial effect of the HA medium was more evident in the group of low-fertility bulls (P < or = 0.03), where neither the variation between bulls (P > or = 0.06) nor the interaction between the methods of sperm selection and bulls (P > or = 0.10) was significant.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:8272535 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8272535 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8272535 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8272535 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8272535 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8272535 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8272535 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8272535 | pubmed:issn | 1031-3613 | lld:pubmed |
| pubmed-article:8272535 | pubmed:author | pubmed-author:LarssonBB | lld:pubmed |
| pubmed-article:8272535 | pubmed:author | pubmed-author:ShamsuddinMM | lld:pubmed |
| pubmed-article:8272535 | pubmed:author | pubmed-author:Rodriguez-Mar... | lld:pubmed |
| pubmed-article:8272535 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8272535 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:8272535 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8272535 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8272535 | pubmed:pagination | 307-15 | lld:pubmed |
| pubmed-article:8272535 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:meshHeading | pubmed-meshheading:8272535-... | lld:pubmed |
| pubmed-article:8272535 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8272535 | pubmed:articleTitle | Fertilizing capacity of bovine spermatozoa selected after swim-up in hyaluronic acid-containing medium. | lld:pubmed |
| pubmed-article:8272535 | pubmed:affiliation | Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala. | lld:pubmed |
| pubmed-article:8272535 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8272535 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8272535 | lld:pubmed |
