| pubmed-article:8245023 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C0011923 | lld:lifeskim |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C0029219 | lld:lifeskim |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C0021547 | lld:lifeskim |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C0444706 | lld:lifeskim |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C1719039 | lld:lifeskim |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:8245023 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:8245023 | pubmed:issue | 34 | lld:pubmed |
| pubmed-article:8245023 | pubmed:dateCreated | 1994-1-4 | lld:pubmed |
| pubmed-article:8245023 | pubmed:abstractText | The distribution and operation of Ca2+ pools within cells has been directly studied in situ by monitoring the Ca2+ inside Ca2+ dye-loaded organelles using high resolution imaging procedures. Using DDT1MF-2 smooth muscle cells, loaded with fura-2 under conditions favoring dye entry into organelles and subjected to carefully controlled permeabilization still attached to coverslips, the Ca2+ within organelles was analyzed by high resolution, z axis-controlled imaging, and deblurring methods. Saturation analysis of entrapped fura-2 indicated that the dye reported Ca2+ identically to fura-2 in solution. Areas containing high Ca(2+)-sequestering organelles (> 5 microM free Ca2+) were observed to predominate around the nucleus and close to the periphery of the cell. Analysis of the actions of inositol 1,4,5-trisphosphate (InsP3) within small (3 microns 2) selected intracellular areas, revealed a "quantal" release phenomenon, with rapid attainment of limited stable release at submaximal InsP3 levels. The apparent EC50 for InsP3 was approximately 3 microns, higher than within suspensions of permeabilized cells. The action of InsP3 was competitively blocked by 10 micrograms/ml of the InsP3 antagonist, heparin. Applied after maximal InsP3-mediated Ca2+ release, heparin reversed InsP3-induced Ca2+ release resulting in reuptake of Ca2+ into Ca(2+)-pumping organelles with identical spatial distribution as before Ca2+ release. InsP3 released Ca2+ from all areas of high Ca(2+)-pumping organelles; extensive areas of high fura-2-loading, but low intraorganelle Ca2+, were unchanged by InsP3. GTP induced no alteration in Ca2+ release (in contrast to suspensions of permeabilized cells), suggesting that the InsP3-sensitive Ca2+ pool was functioning as a single homogeneous pool. Opening of InsP3-sensitive channels was also monitored by assessing InsP3-activated channel-mediated Mn2+ quenching of organelle-loaded fura-2; the results revealed a similar pattern of quantal release, with slightly increased apparent InsP3 sensitivity. The results provide the first high resolution in situ localization of Ca2+ signaling organelles and demonstrate the quantal operation of InsP3-sensitive Ca2+ pools within highly discrete subcellular loci. | lld:pubmed |
| pubmed-article:8245023 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245023 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8245023 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245023 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8245023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8245023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8245023 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8245023 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8245023 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:8245023 | pubmed:author | pubmed-author:GillD LDL | lld:pubmed |
| pubmed-article:8245023 | pubmed:author | pubmed-author:SchneiderM... | lld:pubmed |
| pubmed-article:8245023 | pubmed:author | pubmed-author:KleinM GMG | lld:pubmed |
| pubmed-article:8245023 | pubmed:author | pubmed-author:ShortA DAD | lld:pubmed |
| pubmed-article:8245023 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8245023 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:8245023 | pubmed:volume | 268 | lld:pubmed |
| pubmed-article:8245023 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8245023 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8245023 | pubmed:pagination | 25887-93 | lld:pubmed |
| pubmed-article:8245023 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8245023 | pubmed:meshHeading | pubmed-meshheading:8245023-... | lld:pubmed |
| pubmed-article:8245023 | pubmed:meshHeading | pubmed-meshheading:8245023-... | lld:pubmed |
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| pubmed-article:8245023 | pubmed:meshHeading | pubmed-meshheading:8245023-... | lld:pubmed |
| pubmed-article:8245023 | pubmed:meshHeading | pubmed-meshheading:8245023-... | lld:pubmed |
| pubmed-article:8245023 | pubmed:meshHeading | pubmed-meshheading:8245023-... | lld:pubmed |
| pubmed-article:8245023 | pubmed:meshHeading | pubmed-meshheading:8245023-... | lld:pubmed |
| pubmed-article:8245023 | pubmed:meshHeading | pubmed-meshheading:8245023-... | lld:pubmed |
| pubmed-article:8245023 | pubmed:meshHeading | pubmed-meshheading:8245023-... | lld:pubmed |
| pubmed-article:8245023 | pubmed:meshHeading | pubmed-meshheading:8245023-... | lld:pubmed |
| pubmed-article:8245023 | pubmed:meshHeading | pubmed-meshheading:8245023-... | lld:pubmed |
| pubmed-article:8245023 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8245023 | pubmed:articleTitle | Inositol 1,4,5-trisphosphate-mediated quantal Ca2+ release measured by high resolution imaging of Ca2+ within organelles. | lld:pubmed |
| pubmed-article:8245023 | pubmed:affiliation | Department of Biological Chemistry, University of Maryland School of Medicine, Baltimore 21201. | lld:pubmed |
| pubmed-article:8245023 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8245023 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8245023 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8245023 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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