| pubmed-article:8230324 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8230324 | lifeskim:mentions | umls-concept:C0521009 | lld:lifeskim |
| pubmed-article:8230324 | lifeskim:mentions | umls-concept:C0003069 | lld:lifeskim |
| pubmed-article:8230324 | lifeskim:mentions | umls-concept:C0027836 | lld:lifeskim |
| pubmed-article:8230324 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:8230324 | lifeskim:mentions | umls-concept:C0927232 | lld:lifeskim |
| pubmed-article:8230324 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:8230324 | lifeskim:mentions | umls-concept:C0005220 | lld:lifeskim |
| pubmed-article:8230324 | lifeskim:mentions | umls-concept:C1704711 | lld:lifeskim |
| pubmed-article:8230324 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8230324 | pubmed:dateCreated | 1993-12-7 | lld:pubmed |
| pubmed-article:8230324 | pubmed:abstractText | Bacterial beta-galactosidase is widely used as a marker for gene expression and in cell tracing experiments. In a survey of three transgenic mouse lines expressing beta-galactosidase in the central nervous system (CNS) under the control of different promoters, we find substantial variation in the intracellular distribution of the lacZ protein. In line M beta P5, transgene beta-galactosidase expression is driven by a promoter/enhancer fragment from the oligodendrocyte-specific myelin basic protein gene; however, electron microscopy of histochemically stained preparations reveals transgene expression not only in oligodendrocytes but also in some neurons. Immunofluorescence and immunoperoxidase staining show the beta-galactosidase protein distributed throughout the perikaryal cytoplasm of oligodendrocytes and in processes reaching to myelin sheaths. By contrast, immunoreactive protein appears restricted in neurons to one or a few small perikaryal immunoreactive granules. The granules are visible in the electron microscope as amorphous inclusion bodies of moderate electron density and lack a limiting membrane. Histochemical staining patterns with X-gal and Bluo-gal echoed the protein distribution: diffuse distribution of enzyme protein yielded cells filled with substrate, while punctate enzyme distribution yielded restricted or punctate histochemical staining. Examination of two other lines using different promoter/enhancers to drive expression in the CNS showed both diffuse and punctate beta-galactosidase immunolocalization and histochemical staining. The amount of protein synthesized or other properties, yet unidentified, intrinsic to the target cells may determine the intracellular distribution of beta-galactosidase. In retroviral marking studies, clone members have been identified as those cells filled with X-gal reaction product. This approach may underestimate both clone size and the minimum number of divisions separating the members of each clone. | lld:pubmed |
| pubmed-article:8230324 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8230324 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8230324 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8230324 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8230324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8230324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8230324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8230324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8230324 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8230324 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:8230324 | pubmed:issn | 0360-4012 | lld:pubmed |
| pubmed-article:8230324 | pubmed:author | pubmed-author:LazzariniR... | lld:pubmed |
| pubmed-article:8230324 | pubmed:author | pubmed-author:FriedrichV... | lld:pubmed |
| pubmed-article:8230324 | pubmed:author | pubmed-author:HolsteinG RGR | lld:pubmed |
| pubmed-article:8230324 | pubmed:author | pubmed-author:LULL | lld:pubmed |
| pubmed-article:8230324 | pubmed:author | pubmed-author:GowAA | lld:pubmed |
| pubmed-article:8230324 | pubmed:author | pubmed-author:KelleyK AKA | lld:pubmed |
| pubmed-article:8230324 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8230324 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8230324 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:8230324 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8230324 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8230324 | pubmed:pagination | 88-98 | lld:pubmed |
| pubmed-article:8230324 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:8230324 | pubmed:meshHeading | pubmed-meshheading:8230324-... | lld:pubmed |
| pubmed-article:8230324 | pubmed:meshHeading | pubmed-meshheading:8230324-... | lld:pubmed |
| pubmed-article:8230324 | pubmed:meshHeading | pubmed-meshheading:8230324-... | lld:pubmed |
| pubmed-article:8230324 | pubmed:meshHeading | pubmed-meshheading:8230324-... | lld:pubmed |
| pubmed-article:8230324 | pubmed:meshHeading | pubmed-meshheading:8230324-... | lld:pubmed |
| pubmed-article:8230324 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8230324 | pubmed:articleTitle | Intracellular distribution of transgenic bacterial beta-galactosidase in central nervous system neurons and neuroglia. | lld:pubmed |
| pubmed-article:8230324 | pubmed:affiliation | Brookdale Center for Molecular Biology, Mount Sinai School of Medicine, New York, New York 10029-6574. | lld:pubmed |
| pubmed-article:8230324 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8230324 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8230324 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8230324 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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