| pubmed-article:822842 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:822842 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:822842 | lifeskim:mentions | umls-concept:C0175674 | lld:lifeskim |
| pubmed-article:822842 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:822842 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:822842 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:822842 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:822842 | pubmed:dateCreated | 1976-10-29 | lld:pubmed |
| pubmed-article:822842 | pubmed:abstractText | A novel manual method for protein-sequence analysis is described. Three peptides, the hexapeptide (Leu-TRP-Met-Arg-Phe-Ala), insulin A chain and glucagon were used to test this technique. Peptides (1 or 2 nmol) were hydrolysed with acid and their qualitative amino acid compositions were confirmed by reacting with 4-NN-dimethylaminoazobenzene-4'-sulphonylchloride and 4-NN-dimethylaminoazobenzene 4'-isothiocyanate. Sequence determination of 20-200 nmol of peptide was then performed by the combined use of phenyl isothiocyanate and 4-NN-dimethylaminoazobenzene 4'-isothiocyanate, a new procedure that is analogous to the dansyl-Edman method with the replacement of dansyl chloride by 4-NN-dimethylaminoazobenzene 4'-isothiocyanate as the N-terminal residue determination reagent. On t.l.c. this new N-terminal reagent gave brightly coloured 4-NN-dimethylaminoazobenzene-4-thiohydantoins of amino acids and showed the following advantages: (1) the detection sensitivity is in the pmol range; (2) u.v. observation is not required; (3) there is no destruction of acid-labile amino acids; (4) two-dimensional t.l.c. separation is adequate to identify 24 amino acids, except leucine and isoleucine (this pair of amino acids can be resolved by using 4-NN-dimethylaminoazobenzene-4'-sulphonyl chloride); (5) the determination of a new N-terminal residue (from coupling to t.l.c. identification) takes only 3 h; (6) the colour difference beteen isothiocyanate, thiocarbamoyl and thiohydantoin derivatives facilitates the identifications. | lld:pubmed |
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| pubmed-article:822842 | pubmed:language | eng | lld:pubmed |
| pubmed-article:822842 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:822842 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:822842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:822842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:822842 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:822842 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:822842 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:822842 | pubmed:author | pubmed-author:CreaserE HEH | lld:pubmed |
| pubmed-article:822842 | pubmed:author | pubmed-author:ChangJ YJY | lld:pubmed |
| pubmed-article:822842 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:822842 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:822842 | pubmed:volume | 157 | lld:pubmed |
| pubmed-article:822842 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:822842 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:822842 | pubmed:pagination | 77-85 | lld:pubmed |
| pubmed-article:822842 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:822842 | pubmed:meshHeading | pubmed-meshheading:822842-C... | lld:pubmed |
| pubmed-article:822842 | pubmed:year | 1976 | lld:pubmed |
| pubmed-article:822842 | pubmed:articleTitle | A novel manual method for protein-sequence analysis. | lld:pubmed |
| pubmed-article:822842 | pubmed:publicationType | Journal Article | lld:pubmed |
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