| pubmed-article:8228217 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8228217 | lifeskim:mentions | umls-concept:C0012155 | lld:lifeskim |
| pubmed-article:8228217 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:8228217 | lifeskim:mentions | umls-concept:C0014898 | lld:lifeskim |
| pubmed-article:8228217 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:8228217 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:8228217 | lifeskim:mentions | umls-concept:C1518062 | lld:lifeskim |
| pubmed-article:8228217 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:8228217 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:8228217 | pubmed:dateCreated | 1993-12-10 | lld:pubmed |
| pubmed-article:8228217 | pubmed:abstractText | To precisely determine the biologic role of fish oil-derived constituents (n-3 polyunsaturated fatty acids), it is imperative that information on highly purified n-3 polyunsaturated fatty acids be evolved. Therefore, we studied the effects of a low dose, short term dietary supplementation with highly purified (n-3) ethyl esters with regard to murine T lymphocyte function. A 10-day dietary supplementation with low dose, highly purified (n-3) fatty acid ethyl esters was examined for effects on murine splenic lymphocyte function and membrane composition. Mice were fed diets containing either 3% safflower oil (SAF) ethyl esters, 2% SAF plus 1% eicosapentaenoic acid ethyl esters (EPA) (99% pure), or 2% SAF plus 1% docosahexaenoic acid ethyl esters (DHA) (97% pure). Fatty acid analysis of the lymphocyte membranes showed that membrane compositions of the EPA- and DHA-fed mice were subsequently enriched with these (n-3) fatty acids. Con A-induced lymphoproliferative assays (Con A, 5 and 10 micrograms/ml) revealed that splenic lymphocytes from the EPA group had significantly higher mitogenic responses relative to lymphocytes from the DHA or SAF groups (p < 0.05). In contrast, DHA splenocytes had the highest level of 3H-labeled diradylglycerol as a percentage of total lipid. Macrophage-lymphocyte co-cultures demonstrated that the dietary effect on proliferation in response to Con A was influenced by lymphocyte source, but apparently not by macrophage source. In addition, cells from Mycobacterium bovis vaccine-vaccinated mice were placed in culture with purified protein derivative (PPD) (10, 20, and 40 micrograms/ml), as well as Con A (5 and 10 micrograms/ml) to allow comparison between mitogenic and antigenic stimulation. No statistically significant differences (p > 0.05) existed between the diet groups upon PPD stimulation. However, lymphocytes from the DHA group exhibited significantly higher proliferative responses to Con A than cells from the EPA and SAF groups (p < 0.05). M. bovis vaccine-immunized EPA and DHA fed mice also exhibited significantly reduced delayed-type hypersensitive reactivity to footpad testing with PPD in comparison to that demonstrated by SAF-fed mice (p < 0.05). EPA-fed mice demonstrated the most decreased response. Overall, this study demonstrates for the first time that: 1) low dose, short term dietary supplementation with highly purified EPA or DHA can modulate select functional and transmembrane signaling responses of murine splenic lymphocytes; and 2) that these dietary agents have differing effects on mitogen vs Ag receptors. | lld:pubmed |
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| pubmed-article:8228217 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8228217 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8228217 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:8228217 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8228217 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8228217 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8228217 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:8228217 | pubmed:author | pubmed-author:McMurrayD NDN | lld:pubmed |
| pubmed-article:8228217 | pubmed:author | pubmed-author:ChapkinR SRS | lld:pubmed |
| pubmed-article:8228217 | pubmed:author | pubmed-author:FowlerK HKH | lld:pubmed |
| pubmed-article:8228217 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8228217 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8228217 | pubmed:volume | 151 | lld:pubmed |
| pubmed-article:8228217 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8228217 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8228217 | pubmed:pagination | 5186-97 | lld:pubmed |
| pubmed-article:8228217 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:8228217 | pubmed:meshHeading | pubmed-meshheading:8228217-... | lld:pubmed |
| pubmed-article:8228217 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8228217 | pubmed:articleTitle | Effects of purified dietary n-3 ethyl esters on murine T lymphocyte function. | lld:pubmed |
| pubmed-article:8228217 | pubmed:affiliation | Department of Animal Science, Texas A&M University, College Station 77843-2471. | lld:pubmed |
| pubmed-article:8228217 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8228217 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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