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pubmed-article:8220456pubmed:abstractTextFor plant genetics, it would be useful to monitor easily the segregation of different alleles using the polymerase chain reaction (PCR). Preparation of DNA templates from individual plants needs to be rapid and reliable. A one tube protocol is described that involves subjecting plant tissue pieces to alkali, neutralization and heat denaturation prior to PCR analysis, and that proved to be much faster and more reliable than published protocols.lld:pubmed
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pubmed-article:8220456pubmed:authorpubmed-author:CarrollB JBJlld:pubmed
pubmed-article:8220456pubmed:authorpubmed-author:JonesJ DJDlld:pubmed
pubmed-article:8220456pubmed:authorpubmed-author:ThomasC MCMlld:pubmed
pubmed-article:8220456pubmed:authorpubmed-author:KlimyukV IVIlld:pubmed
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pubmed-article:8220456pubmed:pagination493-4lld:pubmed
pubmed-article:8220456pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:8220456pubmed:articleTitleAlkali treatment for rapid preparation of plant material for reliable PCR analysis.lld:pubmed
pubmed-article:8220456pubmed:affiliationSainsbury Laboratory, John Innes Centre, Norwich, UK.lld:pubmed
pubmed-article:8220456pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8220456pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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