| pubmed-article:8214576 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8214576 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
| pubmed-article:8214576 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:8214576 | lifeskim:mentions | umls-concept:C0596383 | lld:lifeskim |
| pubmed-article:8214576 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8214576 | pubmed:dateCreated | 1993-11-16 | lld:pubmed |
| pubmed-article:8214576 | pubmed:abstractText | Phosphodiester-oligonucleotides were separated by ion-pair reversed-phase HPLC on nonporous polystyrene-based particles having a diameter of 2.1 +/- 0.12 microns. With unmodified poly(styrene-divinylbenzene) beads it was not possible to resolve oligonucleotides efficiently. However, upon addition of polyvinyl alcohol during polymerization baseline resolution of phosphorylated oligodeoxyadenylic acids with a chain length of up to 30 bases was obtained, with triethylammonium acetate serving as ion-pairing reagent. An even higher separation efficiency was achieved by Friedel-Crafts alkylation of poly(styrene-divinylbenzene) particles. At a column temperature of 50 degrees C, the number of theoretical plates exceeded 6 x 10(5) per meter. The maximum loading capacity for a 50 x 4.6-mm i.d. column still resulting in the highest resolution was 0.1 microgram. Calibration curves showed excellent linearity over a range of at least 4 magnitudes, with a lower mass detection limit of 0.16 ng. Recoveries ranged from 96.7 to 100.8%. The same stationary phase also allowed the separation of phosphorylated from dephosphorylated oligonucleotides, the former ones being eluted earlier. | lld:pubmed |
| pubmed-article:8214576 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8214576 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8214576 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8214576 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8214576 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8214576 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8214576 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8214576 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8214576 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:8214576 | pubmed:author | pubmed-author:BonnG KGK | lld:pubmed |
| pubmed-article:8214576 | pubmed:author | pubmed-author:OefnerP JPJ | lld:pubmed |
| pubmed-article:8214576 | pubmed:author | pubmed-author:HuberC GCG | lld:pubmed |
| pubmed-article:8214576 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8214576 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8214576 | pubmed:volume | 212 | lld:pubmed |
| pubmed-article:8214576 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8214576 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8214576 | pubmed:pagination | 351-8 | lld:pubmed |
| pubmed-article:8214576 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8214576 | pubmed:meshHeading | pubmed-meshheading:8214576-... | lld:pubmed |
| pubmed-article:8214576 | pubmed:meshHeading | pubmed-meshheading:8214576-... | lld:pubmed |
| pubmed-article:8214576 | pubmed:meshHeading | pubmed-meshheading:8214576-... | lld:pubmed |
| pubmed-article:8214576 | pubmed:meshHeading | pubmed-meshheading:8214576-... | lld:pubmed |
| pubmed-article:8214576 | pubmed:meshHeading | pubmed-meshheading:8214576-... | lld:pubmed |
| pubmed-article:8214576 | pubmed:meshHeading | pubmed-meshheading:8214576-... | lld:pubmed |
| pubmed-article:8214576 | pubmed:meshHeading | pubmed-meshheading:8214576-... | lld:pubmed |
| pubmed-article:8214576 | pubmed:meshHeading | pubmed-meshheading:8214576-... | lld:pubmed |
| pubmed-article:8214576 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8214576 | pubmed:articleTitle | High-resolution liquid chromatography of oligonucleotides on nonporous alkylated styrene-divinylbenzene copolymers. | lld:pubmed |
| pubmed-article:8214576 | pubmed:affiliation | Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens-University, Innsbruck, Austria. | lld:pubmed |
| pubmed-article:8214576 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8214576 | pubmed:publicationType | Comparative Study | lld:pubmed |
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