| pubmed-article:8195679 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8195679 | lifeskim:mentions | umls-concept:C0014792 | lld:lifeskim |
| pubmed-article:8195679 | lifeskim:mentions | umls-concept:C0596901 | lld:lifeskim |
| pubmed-article:8195679 | lifeskim:mentions | umls-concept:C0059249 | lld:lifeskim |
| pubmed-article:8195679 | lifeskim:mentions | umls-concept:C0031676 | lld:lifeskim |
| pubmed-article:8195679 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:8195679 | lifeskim:mentions | umls-concept:C0205307 | lld:lifeskim |
| pubmed-article:8195679 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:8195679 | lifeskim:mentions | umls-concept:C1704970 | lld:lifeskim |
| pubmed-article:8195679 | lifeskim:mentions | umls-concept:C0332514 | lld:lifeskim |
| pubmed-article:8195679 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:8195679 | pubmed:dateCreated | 1994-6-29 | lld:pubmed |
| pubmed-article:8195679 | pubmed:abstractText | We investigated the use of annexin V (placental anticoagulant protein I), a calcium-dependent protein that binds tightly to phosphatidylserine-containing membranes, as a means to measure membrane phospholipid asymmetry in human erythrocytes. Iodine 125-labeled annexin V bound to erythrocytes in a specific, reversible, calcium-dependent reaction (dissociation constant = 25 +/- 4 nmol/L at 37 degrees C and 2.5 mmol/L calcium). Lysed erythrocytes contained 1.2 x 10(6) binding sites for annexin V. Treatment of erythrocytes with 1 mumol/L A23187 in the presence of 2.5 mmol/L calcium at 37 degrees C caused a gradual but marked increase in annexin V binding sites, reaching a level of approximately 300,000 sites per cell after 8 hours of incubation. We also noted a very gradual spontaneous exposure of annexin V binding sites during storage of purified erythrocytes, reaching a level of approximately 20,000 sites per cell after 30 days. Measurements could also be made directly on diluted whole blood specimens. In samples freshly drawn from 35 normal donors, a mean number of 276 sites per cell were present; this increased to 858 sites per cell after storage of specimens at 4 degrees C for 24 hours. Measurement of annexin V binding to samples from patients with sickle-cell anemia revealed a marked increase in binding (mean of 12,430 sites per cell for all samples); serial measurements in a patient hospitalized with sickle-cell crisis showed a progressive decline in annexin V binding over a period of 6 days.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:8195679 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195679 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195679 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8195679 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195679 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:8195679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195679 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8195679 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8195679 | pubmed:month | May | lld:pubmed |
| pubmed-article:8195679 | pubmed:issn | 0022-2143 | lld:pubmed |
| pubmed-article:8195679 | pubmed:author | pubmed-author:GibsonDD | lld:pubmed |
| pubmed-article:8195679 | pubmed:author | pubmed-author:TaitJ FJF | lld:pubmed |
| pubmed-article:8195679 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8195679 | pubmed:volume | 123 | lld:pubmed |
| pubmed-article:8195679 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8195679 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8195679 | pubmed:pagination | 741-8 | lld:pubmed |
| pubmed-article:8195679 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:meshHeading | pubmed-meshheading:8195679-... | lld:pubmed |
| pubmed-article:8195679 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8195679 | pubmed:articleTitle | Measurement of membrane phospholipid asymmetry in normal and sickle-cell erythrocytes by means of annexin V binding. | lld:pubmed |
| pubmed-article:8195679 | pubmed:affiliation | Department of Laboratory Medicine, University of Washington, Seattle 98195. | lld:pubmed |
| pubmed-article:8195679 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8195679 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8195679 | lld:pubmed |
