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pubmed-article:8182756pubmed:abstractTextSingle crystals of the lipase from Bacillus subtilis have been obtained using a mixture of polyethylene glycol 4000 and sodium sulphate solution as the precipitant. The crystals grow at room temperature in two to three weeks in the presence of n-octyl-beta-D-glucoside. They belong to the monoclinic space group C2 with a = 121.20 A, b = 93.19 A, c = 80.96 A, and beta = 110.67 degrees, with four protein molecules per asymmetric unit. The crystals diffract to at least 2.5 A resolution and are suitable for an X-ray structure analysis.lld:pubmed
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pubmed-article:8182756pubmed:monthMaylld:pubmed
pubmed-article:8182756pubmed:issn0022-2836lld:pubmed
pubmed-article:8182756pubmed:authorpubmed-author:ColsonCClld:pubmed
pubmed-article:8182756pubmed:authorpubmed-author:DijkstraB WBWlld:pubmed
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pubmed-article:8182756pubmed:pagination857-9lld:pubmed
pubmed-article:8182756pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:8182756pubmed:year1994lld:pubmed
pubmed-article:8182756pubmed:articleTitleCrystallization and preliminary X-ray analysis of a lipase from Bacillus subtilis.lld:pubmed
pubmed-article:8182756pubmed:affiliationLaboratory of Biophysical Chemistry, University of Groningen, The Netherlands.lld:pubmed
pubmed-article:8182756pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8182756pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed