| pubmed-article:8179187 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8179187 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8179187 | lifeskim:mentions | umls-concept:C1704336 | lld:lifeskim |
| pubmed-article:8179187 | lifeskim:mentions | umls-concept:C0016223 | lld:lifeskim |
| pubmed-article:8179187 | lifeskim:mentions | umls-concept:C0027270 | lld:lifeskim |
| pubmed-article:8179187 | lifeskim:mentions | umls-concept:C0242485 | lld:lifeskim |
| pubmed-article:8179187 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:8179187 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:8179187 | lifeskim:mentions | umls-concept:C0303920 | lld:lifeskim |
| pubmed-article:8179187 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8179187 | pubmed:dateCreated | 1994-6-9 | lld:pubmed |
| pubmed-article:8179187 | pubmed:abstractText | Saponin-skinned human muscle fibers from M. vastus lateralis were immobilized in a quartz capillary to detect the fluorescence changes of NAD(P)H and of fluorescent flavoproteins. To get sufficient intense fluorescence signals from a small amount of muscle tissue the NAD(P)H fluorescence was excited by means of an HeCd laser at 325 nm and the flavoprotein fluorescence by an argon-ion laser at 454 nm or by the second wavelength of a HeCd laser at 442 nm. Using this experimental setup the fluorescence spectra of NAD(P)H, of alpha-lipoamide dehydrogenase and of electron-transfer flavoprotein were detected in saponin-skinned human muscle fibers. These fibers behaved identically to isolated mitochondria: (i) The addition of substrates caused an increase in reduction of mitochondrial NAD+, (ii) the addition of ADP caused its reoxidation, and (iii) the addition of respiratory chain inhibitors led to an almost complete reduction of NAD+. It was observed that the redox state of the NAD(P) system and of the alpha-lipoamide dehydrogenase reached after addition of 1 mM ADP correlates with the rate of active state respiration with NAD-dependent substrates. Therefore, this fluorimetric method is suitable to compare the mitochondrial oxidation capacities of NAD-dependent substrates in less then 5 mg wet weight muscle tissue. Moreover, the maximal changes in fluorescence of NAD(P)H and flavoproteins correlate with the amount of mitochondrial marker enzymes per milligram muscle tissue. Using this method a myopathy caused by a diminished content of mitochondria per milligram muscle tissue was observed. | lld:pubmed |
| pubmed-article:8179187 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8179187 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8179187 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8179187 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8179187 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8179187 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8179187 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8179187 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8179187 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8179187 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8179187 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:8179187 | pubmed:author | pubmed-author:WinklerKK | lld:pubmed |
| pubmed-article:8179187 | pubmed:author | pubmed-author:KuznetsovA... | lld:pubmed |
| pubmed-article:8179187 | pubmed:author | pubmed-author:NeumannH WHW | lld:pubmed |
| pubmed-article:8179187 | pubmed:author | pubmed-author:GellerichF... | lld:pubmed |
| pubmed-article:8179187 | pubmed:author | pubmed-author:KunzW SWS | lld:pubmed |
| pubmed-article:8179187 | pubmed:author | pubmed-author:NeuhofSS | lld:pubmed |
| pubmed-article:8179187 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8179187 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8179187 | pubmed:volume | 216 | lld:pubmed |
| pubmed-article:8179187 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8179187 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8179187 | pubmed:pagination | 322-7 | lld:pubmed |
| pubmed-article:8179187 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8179187 | pubmed:meshHeading | pubmed-meshheading:8179187-... | lld:pubmed |
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| pubmed-article:8179187 | pubmed:meshHeading | pubmed-meshheading:8179187-... | lld:pubmed |
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| pubmed-article:8179187 | pubmed:meshHeading | pubmed-meshheading:8179187-... | lld:pubmed |
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| pubmed-article:8179187 | pubmed:meshHeading | pubmed-meshheading:8179187-... | lld:pubmed |
| pubmed-article:8179187 | pubmed:meshHeading | pubmed-meshheading:8179187-... | lld:pubmed |
| pubmed-article:8179187 | pubmed:meshHeading | pubmed-meshheading:8179187-... | lld:pubmed |
| pubmed-article:8179187 | pubmed:meshHeading | pubmed-meshheading:8179187-... | lld:pubmed |
| pubmed-article:8179187 | pubmed:meshHeading | pubmed-meshheading:8179187-... | lld:pubmed |
| pubmed-article:8179187 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8179187 | pubmed:articleTitle | Measurement of fluorescence changes of NAD(P)H and of fluorescent flavoproteins in saponin-skinned human skeletal muscle fibers. | lld:pubmed |
| pubmed-article:8179187 | pubmed:affiliation | Institut für Biochemie, Medizinische Akademie Magdeburg, Germany. | lld:pubmed |
| pubmed-article:8179187 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8179187 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8179187 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8179187 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8179187 | lld:pubmed |
