Linked Life Data

8175719

Source:http://linkedlifedata.com/resource/pubmed/id/8175719

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:8175719rdf:typepubmed:Citationlld:pubmed
pubmed-article:8175719lifeskim:mentionsumls-concept:C0020792lld:lifeskim
pubmed-article:8175719lifeskim:mentionsumls-concept:C0567416lld:lifeskim
pubmed-article:8175719lifeskim:mentionsumls-concept:C0521390lld:lifeskim
pubmed-article:8175719lifeskim:mentionsumls-concept:C0034800lld:lifeskim
pubmed-article:8175719lifeskim:mentionsumls-concept:C0167183lld:lifeskim
pubmed-article:8175719lifeskim:mentionsumls-concept:C0204727lld:lifeskim
pubmed-article:8175719lifeskim:mentionsumls-concept:C0205409lld:lifeskim
pubmed-article:8175719lifeskim:mentionsumls-concept:C0084084lld:lifeskim
pubmed-article:8175719lifeskim:mentionsumls-concept:C1148608lld:lifeskim
pubmed-article:8175719lifeskim:mentionsumls-concept:C1419893lld:lifeskim
pubmed-article:8175719pubmed:issue17lld:pubmed
pubmed-article:8175719pubmed:dateCreated1994-6-6lld:pubmed
pubmed-article:8175719pubmed:abstractTextHB-GAM (heparin binding growth-associated molecule; pleiotrophin) is a secretory, extracellular matrix-associated protein that is strongly expressed in developing nervous tissues and belongs to a novel family of differentiation/growth factors. It promotes axonal growth from perinatal rat brain neurons and is suggested to be mitogenic for some cell types and to display cell-transforming activity. Since the receptors of HB-GAM in cells are unknown, we have started isolation of putative cell surface receptors from brain neurons and from perinatal rat brain. For this purpose, recombinant HB-GAM was produced with the aid of a baculovirus vector and used as an affinity matrix in receptor isolation. A detergent-solubilized component from cultured brain neurons and from brain was identified that binds specifically to HB-GAM and migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a broad smear with an apparent molecular mass of about 200 kDa. This cell surface component was found to contain heparan sulfate chains, which are bound to a core protein with an apparent molecular mass of 120 kDa. Gel electrophoretic characteristics, immunochemical analysis, and partial peptide sequencing revealed that the cell surface component isolated as an HB-GAM receptor is N-syndecan (syndecan-3). In a solid phase binding assay, N-syndecan was found to bind to HB-GAM in a similar manner as to basic fibroblast growth factor (KD = 0.6 nM). Immunofluorescence microscopy indicated that in brain neurons, N-syndecan occurs at the surface of the cell soma and of the neurites that grow along HB-GAM-coated substrates. Anti-N-syndecan antibodies added to culture media had an inhibitory effect on HB-GAM-induced neurite outgrowth. We suggest that N-syndecan mediates the neurite outgrowth-promoting signal from HB-GAM to the cytoskeleton of growing neurites.lld:pubmed
pubmed-article:8175719pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:languageenglld:pubmed
pubmed-article:8175719pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:citationSubsetIMlld:pubmed
pubmed-article:8175719pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8175719pubmed:statusMEDLINElld:pubmed
pubmed-article:8175719pubmed:monthAprlld:pubmed
pubmed-article:8175719pubmed:issn0021-9258lld:pubmed
pubmed-article:8175719pubmed:authorpubmed-author:RauvalaHHlld:pubmed
pubmed-article:8175719pubmed:authorpubmed-author:NomiKKlld:pubmed
pubmed-article:8175719pubmed:authorpubmed-author:RaultBBlld:pubmed
pubmed-article:8175719pubmed:authorpubmed-author:CareyD JDJlld:pubmed
pubmed-article:8175719pubmed:authorpubmed-author:ChernousovM...lld:pubmed
pubmed-article:8175719pubmed:issnTypePrintlld:pubmed
pubmed-article:8175719pubmed:day29lld:pubmed
pubmed-article:8175719pubmed:volume269lld:pubmed
pubmed-article:8175719pubmed:ownerNLMlld:pubmed
pubmed-article:8175719pubmed:authorsCompleteYlld:pubmed
pubmed-article:8175719pubmed:pagination12999-3004lld:pubmed
pubmed-article:8175719pubmed:dateRevised2007-11-14lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:meshHeadingpubmed-meshheading:8175719-...lld:pubmed
pubmed-article:8175719pubmed:year1994lld:pubmed
pubmed-article:8175719pubmed:articleTitleIsolation of a neuronal cell surface receptor of heparin binding growth-associated molecule (HB-GAM). Identification as N-syndecan (syndecan-3).lld:pubmed
pubmed-article:8175719pubmed:affiliationLaboratory of Molecular Neurobiology, University of Helsinki, Finland.lld:pubmed
pubmed-article:8175719pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8175719pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:8175719pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
entrez-gene:6382entrezgene:pubmedpubmed-article:8175719lld:entrezgene
entrez-gene:9672entrezgene:pubmedpubmed-article:8175719lld:entrezgene
entrez-gene:5764entrezgene:pubmedpubmed-article:8175719lld:entrezgene
http://linkedlifedata.com/r...entrezgene:pubmedpubmed-article:8175719lld:entrezgene
http://linkedlifedata.com/r...entrezgene:pubmedpubmed-article:8175719lld:entrezgene
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8175719lld:pubmed