| pubmed-article:8152244 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8152244 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:8152244 | lifeskim:mentions | umls-concept:C0019564 | lld:lifeskim |
| pubmed-article:8152244 | lifeskim:mentions | umls-concept:C0205178 | lld:lifeskim |
| pubmed-article:8152244 | lifeskim:mentions | umls-concept:C1517499 | lld:lifeskim |
| pubmed-article:8152244 | lifeskim:mentions | umls-concept:C0392752 | lld:lifeskim |
| pubmed-article:8152244 | lifeskim:mentions | umls-concept:C1519355 | lld:lifeskim |
| pubmed-article:8152244 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8152244 | pubmed:dateCreated | 1994-5-12 | lld:pubmed |
| pubmed-article:8152244 | pubmed:abstractText | Viral vectors derived from herpes simplex virus, type-1 (HSV), can transfer and express genes into fully differentiated, post-mitotic neurons. These vectors also transduce cells effectively in organotypic hippocampal slice cultures. Nanoliter quantities of a virus stock of HSVlac, an HSV vector that directs expression of E. coli beta-galactosidase (beta-gal), were microapplied into stratum pyramidale or stratum granulosum of slice cultures. Twenty-four hours later, a cluster of transduced cells expressing beta-gal was observed at the microapplication site. Gene transfer by microapplication was both effective and rapid. The titer of the HSVlac stocks was determined on NIH3T3 cells. Eighty-three percent of the beta-gal forming units successfully transduced beta-gal after microapplication to slice cultures. beta-Gal expression was detected as rapidly as 4 h after transduction into cultures of fibroblasts or hippocampal slices. The rapid expression of beta-gal by HSVlac allowed efficient transduction of acute hippocampal slices. Many genes have been transduced and expressed using HSV vectors; therefore, this microapplication method can be applied to many neurobiological questions. | lld:pubmed |
| pubmed-article:8152244 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8152244 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8152244 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8152244 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8152244 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8152244 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8152244 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8152244 | pubmed:issn | 0165-0270 | lld:pubmed |
| pubmed-article:8152244 | pubmed:author | pubmed-author:BrownleeMM | lld:pubmed |
| pubmed-article:8152244 | pubmed:author | pubmed-author:EdelsteinDD | lld:pubmed |
| pubmed-article:8152244 | pubmed:author | pubmed-author:ShenHH | lld:pubmed |
| pubmed-article:8152244 | pubmed:author | pubmed-author:FederoffH JHJ | lld:pubmed |
| pubmed-article:8152244 | pubmed:author | pubmed-author:StelzerAA | lld:pubmed |
| pubmed-article:8152244 | pubmed:author | pubmed-author:BergoldP JPJ | lld:pubmed |
| pubmed-article:8152244 | pubmed:author | pubmed-author:BenedikzEE | lld:pubmed |
| pubmed-article:8152244 | pubmed:author | pubmed-author:GeschwindMM | lld:pubmed |
| pubmed-article:8152244 | pubmed:author | pubmed-author:Casaccia-Bonn... | lld:pubmed |
| pubmed-article:8152244 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8152244 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:8152244 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8152244 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8152244 | pubmed:pagination | 341-51 | lld:pubmed |
| pubmed-article:8152244 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:meshHeading | pubmed-meshheading:8152244-... | lld:pubmed |
| pubmed-article:8152244 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8152244 | pubmed:articleTitle | Localized gene transfer into organotypic hippocampal slice cultures and acute hippocampal slices. | lld:pubmed |
| pubmed-article:8152244 | pubmed:affiliation | Program of Anatomy and Cell Biology, State University of New York-Health Science Center at Brooklyn 11203. | lld:pubmed |
| pubmed-article:8152244 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8152244 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:8152244 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8152244 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8152244 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8152244 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8152244 | lld:pubmed |
