| pubmed-article:8143773 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8143773 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:8143773 | lifeskim:mentions | umls-concept:C1512336 | lld:lifeskim |
| pubmed-article:8143773 | lifeskim:mentions | umls-concept:C0385333 | lld:lifeskim |
| pubmed-article:8143773 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8143773 | lifeskim:mentions | umls-concept:C0016263 | lld:lifeskim |
| pubmed-article:8143773 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:8143773 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:8143773 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:8143773 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8143773 | pubmed:dateCreated | 1994-5-3 | lld:pubmed |
| pubmed-article:8143773 | pubmed:abstractText | Using a procedure to stain cells simultaneously for cyclin B1 protein and DNA, we have examined cyclin B1 expression by flow cytometry in human cells under a variety of perturbing and nonperturbing conditions. The method described is useful for measuring relative differences in cyclin B level (immunochemically detectable epitope) as a function of cell cycle position on an individual cell basis and thus to examine cell cycle-related changes in cyclin B expression without prior cell synchronization. We show that in HeLaS3 cells, cyclin B1 accumulates in cells only after they become 4C and have resided in G2 for a short period of time. During colcemid-induced mitotic arrest cyclin B1 continues to accumulate in HeLa S3 cells, and under specific conditions of aphidicolin-induced unbalanced cell growth induced, cyclin B accumulates to supranormal levels prior to mitosis. Flow cytometric analysis of cyclin B expression and DNA content permits detailed examination of the effects of cell cycle perturbations on cyclin B expression under a variety of conditions. | lld:pubmed |
| pubmed-article:8143773 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8143773 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8143773 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8143773 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8143773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8143773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8143773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8143773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8143773 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8143773 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8143773 | pubmed:issn | 0014-4827 | lld:pubmed |
| pubmed-article:8143773 | pubmed:author | pubmed-author:SchimkeR TRT | lld:pubmed |
| pubmed-article:8143773 | pubmed:author | pubmed-author:RushD FDF | lld:pubmed |
| pubmed-article:8143773 | pubmed:author | pubmed-author:SherwoodS WSW | lld:pubmed |
| pubmed-article:8143773 | pubmed:author | pubmed-author:KENTNN | lld:pubmed |
| pubmed-article:8143773 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8143773 | pubmed:volume | 211 | lld:pubmed |
| pubmed-article:8143773 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8143773 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8143773 | pubmed:pagination | 275-81 | lld:pubmed |
| pubmed-article:8143773 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8143773 | pubmed:meshHeading | pubmed-meshheading:8143773-... | lld:pubmed |
| pubmed-article:8143773 | pubmed:meshHeading | pubmed-meshheading:8143773-... | lld:pubmed |
| pubmed-article:8143773 | pubmed:meshHeading | pubmed-meshheading:8143773-... | lld:pubmed |
| pubmed-article:8143773 | pubmed:meshHeading | pubmed-meshheading:8143773-... | lld:pubmed |
| pubmed-article:8143773 | pubmed:meshHeading | pubmed-meshheading:8143773-... | lld:pubmed |
| pubmed-article:8143773 | pubmed:meshHeading | pubmed-meshheading:8143773-... | lld:pubmed |
| pubmed-article:8143773 | pubmed:meshHeading | pubmed-meshheading:8143773-... | lld:pubmed |
| pubmed-article:8143773 | pubmed:meshHeading | pubmed-meshheading:8143773-... | lld:pubmed |
| pubmed-article:8143773 | pubmed:meshHeading | pubmed-meshheading:8143773-... | lld:pubmed |
| pubmed-article:8143773 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8143773 | pubmed:articleTitle | Cyclin B1 expression in HeLa S3 cells studied by flow cytometry. | lld:pubmed |
| pubmed-article:8143773 | pubmed:affiliation | Department of Biological Sciences, Stanford University, California 94305. | lld:pubmed |
| pubmed-article:8143773 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8143773 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8143773 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8143773 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8143773 | lld:pubmed |
