| pubmed-article:8133269 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8133269 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:8133269 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:8133269 | lifeskim:mentions | umls-concept:C0039067 | lld:lifeskim |
| pubmed-article:8133269 | lifeskim:mentions | umls-concept:C0013030 | lld:lifeskim |
| pubmed-article:8133269 | lifeskim:mentions | umls-concept:C0178702 | lld:lifeskim |
| pubmed-article:8133269 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:8133269 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:8133269 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:8133269 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:8133269 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:8133269 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8133269 | pubmed:dateCreated | 1994-4-20 | lld:pubmed |
| pubmed-article:8133269 | pubmed:abstractText | Potential desensitization of brain nicotinic receptors was studied using a [3H]dopamine release assay. Nicotine-stimulated [3H]dopamine release from mouse striatal synaptosomes was concentration-dependent with an EC50 of 0.33 +/- 0.13 microM and a Hill coefficient of 1.44 +/- 0.18. Desensitization by activating concentrations of nicotine had a similar EC50 and a half-time of 35 s. Concentrations of nicotine that evoked little release also induced a concentration-dependent desensitization (EC50 = 6.9 +/- 3.6 nM, t1/2 = 1.6-2.0 min, nH = 1.02 +/- 0.01). Both types of desensitization produced a maximum 75% decrease in [3H]dopamine release. Recovery from desensitization after exposure to low or activating concentrations of nicotine was time-dependent with half-times of 6.1 min and 12.4 min, respectively. Constants determined for binding of [3H]nicotine to striatal membrane at 22 degrees C included a KD of 3.7 +/- 0.5 nM, Bmax of 67.5 +/- 2.2 fmol/mg, and Hill coefficient of 1.07 +/- 0.06. Association of nicotine with membrane binding sites was biphasic with half-times of 9 s and 1.8 min. The fast rate process contributed 37% of the total reaction. Dissociation was a uniphasic process with a half-time of 1.6 min. Comparison of constants determined by the release and binding assays indicated that the [3H]-nicotine binding site could be the presynaptic receptor involved in [3H]dopamine release in mouse striatal synaptosomes. | lld:pubmed |
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| pubmed-article:8133269 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8133269 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8133269 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8133269 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8133269 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8133269 | pubmed:issn | 0022-3042 | lld:pubmed |
| pubmed-article:8133269 | pubmed:author | pubmed-author:CollinsA CAC | lld:pubmed |
| pubmed-article:8133269 | pubmed:author | pubmed-author:MarksM JMJ | lld:pubmed |
| pubmed-article:8133269 | pubmed:author | pubmed-author:GradyS RSR | lld:pubmed |
| pubmed-article:8133269 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8133269 | pubmed:volume | 62 | lld:pubmed |
| pubmed-article:8133269 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8133269 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8133269 | pubmed:pagination | 1390-8 | lld:pubmed |
| pubmed-article:8133269 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:8133269 | pubmed:meshHeading | pubmed-meshheading:8133269-... | lld:pubmed |
| pubmed-article:8133269 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8133269 | pubmed:articleTitle | Desensitization of nicotine-stimulated [3H]dopamine release from mouse striatal synaptosomes. | lld:pubmed |
| pubmed-article:8133269 | pubmed:affiliation | Institute for Behavioral Genetics, University of Colorado, Boulder. | lld:pubmed |
| pubmed-article:8133269 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8133269 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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