pubmed-article:8129710 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8129710 | lifeskim:mentions | umls-concept:C0008051 | lld:lifeskim |
pubmed-article:8129710 | lifeskim:mentions | umls-concept:C1510668 | lld:lifeskim |
pubmed-article:8129710 | lifeskim:mentions | umls-concept:C0009264 | lld:lifeskim |
pubmed-article:8129710 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
pubmed-article:8129710 | lifeskim:mentions | umls-concept:C0917705 | lld:lifeskim |
pubmed-article:8129710 | lifeskim:mentions | umls-concept:C1656379 | lld:lifeskim |
pubmed-article:8129710 | lifeskim:mentions | umls-concept:C0332162 | lld:lifeskim |
pubmed-article:8129710 | pubmed:dateCreated | 1994-4-14 | lld:pubmed |
pubmed-article:8129710 | pubmed:abstractText | The effect of alpha-actinin from chicken gizzard on the properties of F-actin solutions at 37 degrees C and at 4 degrees C was investigated. Beside the well-known increase of the gelling activity of alpha-actinin, it was found that lowering temperature to 4 degrees C: (a) modifies the shape of the alpha-actinin-F-actin binding isotherm; (b) increases the light scattering of the alpha-actinin-F-actin mixtures; (c) induces the formation of ribbons and bundles of F-actin. It was also observed that, by warming to 37 degrees C, the bundles of F-actin formed at 4 degrees C were dissociated into quasi-parallel actin filaments running at a distance of 25-42 nm from each other. On subsequent cooling to 4 degrees C, these parallel filaments were rapidly assembled into bundles. As at 37 degrees C, alpha-actinin displays a potent gelling activity on bundles but not on filaments of actin, and as the gelling activity at 4 degrees C is accompanied by the formation of actin bundles, it is concluded that actin bundling is a necessary condition to promote the actin-gelling activity. | lld:pubmed |
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pubmed-article:8129710 | pubmed:language | eng | lld:pubmed |
pubmed-article:8129710 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8129710 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:8129710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8129710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8129710 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8129710 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8129710 | pubmed:month | Feb | lld:pubmed |
pubmed-article:8129710 | pubmed:issn | 0264-6021 | lld:pubmed |
pubmed-article:8129710 | pubmed:author | pubmed-author:GraziEE | lld:pubmed |
pubmed-article:8129710 | pubmed:author | pubmed-author:MagriEE | lld:pubmed |
pubmed-article:8129710 | pubmed:author | pubmed-author:TrombettaGG | lld:pubmed |
pubmed-article:8129710 | pubmed:author | pubmed-author:CuneoPP | lld:pubmed |
pubmed-article:8129710 | pubmed:author | pubmed-author:Schwienbacher... | lld:pubmed |
pubmed-article:8129710 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8129710 | pubmed:day | 15 | lld:pubmed |
pubmed-article:8129710 | pubmed:volume | 298 ( Pt 1) | lld:pubmed |
pubmed-article:8129710 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8129710 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8129710 | pubmed:pagination | 129-33 | lld:pubmed |
pubmed-article:8129710 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:8129710 | pubmed:year | 1994 | lld:pubmed |
pubmed-article:8129710 | pubmed:articleTitle | alpha-Actinin from chicken gizzard: at low temperature, the onset of actin-gelling activity correlates with actin bundling. | lld:pubmed |
pubmed-article:8129710 | pubmed:affiliation | Instituto di Chimica Biologica, Università di Ferrara, Italy. | lld:pubmed |
pubmed-article:8129710 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:8129710 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |