| pubmed-article:8099075 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8099075 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:8099075 | lifeskim:mentions | umls-concept:C0024369 | lld:lifeskim |
| pubmed-article:8099075 | lifeskim:mentions | umls-concept:C0332208 | lld:lifeskim |
| pubmed-article:8099075 | lifeskim:mentions | umls-concept:C0332466 | lld:lifeskim |
| pubmed-article:8099075 | lifeskim:mentions | umls-concept:C1254042 | lld:lifeskim |
| pubmed-article:8099075 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:8099075 | pubmed:dateCreated | 1993-6-29 | lld:pubmed |
| pubmed-article:8099075 | pubmed:abstractText | Macropinosomes formed by addition of recombinant macrophage colony-stimulating factor (rM-CSF) to mouse macrophages migrate centripetally and shrink, remaining detectable by phase microscopy for up to 15 min. This longevity allowed us to study how macropinosomes age. Macropinosomes were pulse labeled for 1 min with fixable fluorescein dextran (FDx10f), a probe for fluid phase pinocytosis, and chased for various times. To quantify changes in their antigenic profile, pulse-labeled macropinosomes of different ages were fixed and stained for immunofluorescence with a panel of antibodies specific for the transferrin receptor (TfR), the late endosome-specific, GTP-binding protein rab 7 or lysosomal glycoprotein A (lgp-A), and the percentage of antibody positive, FDx10f-labeled macropinosomes was scored. Some newly formed macropinosomes were positive for TfR, but few were rab 7 or lgp-A-positive. With intermediate chase times (2-4 min), staining for rab 7 and lgp-A increased to > 60%, while TfR staining declined. After a long chase (9-12 min), rab 7 staining returned to low levels while lgp-A staining remained at a high level. Thus, macropinosomes matured by progressive acquisition and loss of characteristic endocytic vesicle markers. However, unlike a maturation process, their merger with the tubular lysosomal compartment more nearly resembled the incorporation of a transient vesicle into a pre-existing, stable compartment. Shortly after their formation, FDx10f-labeled macropinosomes contacted and merged with Texas red dextran (TRDx10)-labeled tubular lysosomes. This occurred in two steps: macropinosomes acquired lgp-A first, and then several minutes later the cation-independent mannose-6-phosphate receptor (CI-MPR) and markers of lysosomal content (cathepsin L or pre-loaded TRDx10), all apparently derived from tubular lysosomes. Thus, macropinosome progress through macrophages showed features of both the maturation and vesicle shuttle models of endocytosis, beginning with a maturation process and ending by merger into a stable, resident lysosomal compartment. | lld:pubmed |
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| pubmed-article:8099075 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8099075 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8099075 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8099075 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8099075 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:8099075 | pubmed:issn | 0021-9525 | lld:pubmed |
| pubmed-article:8099075 | pubmed:author | pubmed-author:SwansonJ AJA | lld:pubmed |
| pubmed-article:8099075 | pubmed:author | pubmed-author:RacoosinE LEL | lld:pubmed |
| pubmed-article:8099075 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8099075 | pubmed:volume | 121 | lld:pubmed |
| pubmed-article:8099075 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8099075 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8099075 | pubmed:pagination | 1011-20 | lld:pubmed |
| pubmed-article:8099075 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8099075 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8099075 | pubmed:articleTitle | Macropinosome maturation and fusion with tubular lysosomes in macrophages. | lld:pubmed |
| pubmed-article:8099075 | pubmed:affiliation | Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, Massachusetts 02115. | lld:pubmed |
| pubmed-article:8099075 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8099075 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:8099075 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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