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pubmed-article:8094306pubmed:abstractTextAnalytical methods have been developed for the simultaneous quantitation of azelastine (AZ) and desmethylazelastine (DAZ) in guinea pig plasma and lung tissue. The methods require a 1.00 mL plasma sample and a minimum 0.100 g lung sample. Both methods employ liquid/liquid organic extraction and back-extraction into dilute acid. Quantitation is performed by high performance liquid chromatography on a 2 x 250 mm 5 microns Hypersil CPS column using fluorescence detection. The linear quantitative ranges for AZ.HCl and DAZ.HBr in plasma are 0.156-160 ng/mL and 0.313-160 ng/mL, respectively. The linear quantitative ranges for AZ.HCl and DAZ.HBr in lung tissue are 0.039-20 micrograms/g for both.lld:pubmed
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pubmed-article:8094306pubmed:authorpubmed-author:SofiaR DRDlld:pubmed
pubmed-article:8094306pubmed:authorpubmed-author:KucharczykNNlld:pubmed
pubmed-article:8094306pubmed:authorpubmed-author:WichmannJ KJKlld:pubmed
pubmed-article:8094306pubmed:authorpubmed-author:PivonkaJJlld:pubmed
pubmed-article:8094306pubmed:authorpubmed-author:LangevinC NCNlld:pubmed
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pubmed-article:8094306pubmed:pagination7-11lld:pubmed
pubmed-article:8094306pubmed:dateRevised2004-12-8lld:pubmed
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pubmed-article:8094306pubmed:articleTitleHigh performance liquid chromatographic determination of azelastine and desmethylazelastine in guinea pig plasma and lung tissue.lld:pubmed
pubmed-article:8094306pubmed:affiliationDepartment of Biochemistry, Wallace Laboratories, Division of Carter-Wallace, Inc., Cranbury, NJ 08512.lld:pubmed
pubmed-article:8094306pubmed:publicationTypeJournal Articlelld:pubmed