| pubmed-article:8094102 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8094102 | lifeskim:mentions | umls-concept:C0014609 | lld:lifeskim |
| pubmed-article:8094102 | lifeskim:mentions | umls-concept:C0007603 | lld:lifeskim |
| pubmed-article:8094102 | lifeskim:mentions | umls-concept:C0521378 | lld:lifeskim |
| pubmed-article:8094102 | lifeskim:mentions | umls-concept:C0074254 | lld:lifeskim |
| pubmed-article:8094102 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:8094102 | lifeskim:mentions | umls-concept:C1512948 | lld:lifeskim |
| pubmed-article:8094102 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8094102 | pubmed:dateCreated | 1993-3-11 | lld:pubmed |
| pubmed-article:8094102 | pubmed:abstractText | Secretin is thought to cause choleresis by acting on a receptor expressed by bile duct epithelial cells. In this study, the receptor was characterized using a new preparation of intrahepatic bile duct plasma membranes. Hyperplastic biliary trees were obtained from 3-week bile duct-ligated rats. The biliary trees were homogenized, filtered, and subjected to an aqueous two-phase partition technique to yield highly purified plasma membranes (confirmed by a 14-fold enrichment in gamma-glutamyl transpeptidase activity and a 10-fold enrichment in 125I-secretin binding). 125I-secretin bound saturably with high affinity and in a dose-dependent fashion (Kd = 1.3 +/- 0.1 nM, Bmax = 273 +/- 23 fmole/mg) to purified plasma membranes. The binding characteristics of secretin were most consistent with a single site receptor model. Competitive binding studies indicated that the secretin-related peptides glucagon, peptide histidine isoleucine, gastric inhibitory peptide, and growth hormone releasing factor did not inhibit binding. Vasoactive intestinal peptide (1 microM) reduced maximal binding by 19 +/- 1%. The GTP analogs guanylylimidodiphosphate and guanosine 5'-O-[3-thiotriphosphate] (1 microM) inhibited binding by 16 +/- 2 and 13 +/- 1%, respectively. In conclusion, secretin binds to a specific, high-affinity receptor in intrahepatic bile duct epithelium that is coupled to a G-protein-linked signal transduction system. | lld:pubmed |
| pubmed-article:8094102 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8094102 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8094102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8094102 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8094102 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8094102 | pubmed:issn | 0022-4804 | lld:pubmed |
| pubmed-article:8094102 | pubmed:author | pubmed-author:MeyersW CWC | lld:pubmed |
| pubmed-article:8094102 | pubmed:author | pubmed-author:VignaS RSR | lld:pubmed |
| pubmed-article:8094102 | pubmed:author | pubmed-author:GettysT WTW | lld:pubmed |
| pubmed-article:8094102 | pubmed:author | pubmed-author:McVeyD CDC | lld:pubmed |
| pubmed-article:8094102 | pubmed:author | pubmed-author:FaroukMM | lld:pubmed |
| pubmed-article:8094102 | pubmed:author | pubmed-author:PruthiR SRS | lld:pubmed |
| pubmed-article:8094102 | pubmed:author | pubmed-author:CharaJJ | lld:pubmed |
| pubmed-article:8094102 | pubmed:author | pubmed-author:HaebigJ EJE | lld:pubmed |
| pubmed-article:8094102 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8094102 | pubmed:volume | 54 | lld:pubmed |
| pubmed-article:8094102 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8094102 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8094102 | pubmed:pagination | 1-6 | lld:pubmed |
| pubmed-article:8094102 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8094102 | pubmed:meshHeading | pubmed-meshheading:8094102-... | lld:pubmed |
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| pubmed-article:8094102 | pubmed:meshHeading | pubmed-meshheading:8094102-... | lld:pubmed |
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| pubmed-article:8094102 | pubmed:meshHeading | pubmed-meshheading:8094102-... | lld:pubmed |
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| pubmed-article:8094102 | pubmed:meshHeading | pubmed-meshheading:8094102-... | lld:pubmed |
| pubmed-article:8094102 | pubmed:meshHeading | pubmed-meshheading:8094102-... | lld:pubmed |
| pubmed-article:8094102 | pubmed:meshHeading | pubmed-meshheading:8094102-... | lld:pubmed |
| pubmed-article:8094102 | pubmed:meshHeading | pubmed-meshheading:8094102-... | lld:pubmed |
| pubmed-article:8094102 | pubmed:meshHeading | pubmed-meshheading:8094102-... | lld:pubmed |
| pubmed-article:8094102 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8094102 | pubmed:articleTitle | Secretin receptors in a new preparation of plasma membranes from intrahepatic biliary epithelium. | lld:pubmed |
| pubmed-article:8094102 | pubmed:affiliation | Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710. | lld:pubmed |
| pubmed-article:8094102 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8094102 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8094102 | lld:pubmed |
