| pubmed-article:809280 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:809280 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:809280 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:809280 | lifeskim:mentions | umls-concept:C1700775 | lld:lifeskim |
| pubmed-article:809280 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:809280 | pubmed:dateCreated | 1975-12-29 | lld:pubmed |
| pubmed-article:809280 | pubmed:abstractText | 1. Monomeric nu-chains were conjugated with CNBr-activated Sepharose 4B. The C-terminal cysteine of the conjugated nu-chain was converted to a mixed disulfide with 3-carboxy-4-nitro-benzenethiol (Nbs) and used to separate plasma proteins with reactive thiol groups. The plasma proteins, alpha1-antitrypsin and prealbumin have the greatest affinity for the interchange reaction with mixed disulfides. The disulfide link between alpha1-antitrypsin and nu-chain is sensitive to excess Nbs, and is selectively cleaved in the presence of 5,5'-dithiobis(2-nitrobenzoate) (Nbs2) which accepts the sulfhydryl group of alpha1-antitrypsin. 2. A Simple method developed for the isolation of human alpha1-antitrypsin was equally effective for the various inherited phenotypes and for alpha1-antitrypsin from the dog, baboon, and monkey, Glutathione-Sepharose was also used successfully, but the nu-chain conjugate yielded alpha1-antitrypsin less contaminated with mercaptalbumin and prealbumin. 3. The alpha1-antitrypsin is harvested from this procedure as a mixed disulfide with Nbs. The negative charge of Nbs at pH 8.1 causes an increased electrophoretic mobility of the alpha1-antitrypsin derivative. Mild reduction liberates Nbs and electrophoretic mobility of alpha1-antitrypsin returns to normal. The method described can increase the alpha1-antitrypsin content of a plasma fraction from 5% of the total protein to 95% within one day with a yield of about 50%. This purification procedure does not exert any detectable effect on microheterogeneity. | lld:pubmed |
| pubmed-article:809280 | pubmed:language | eng | lld:pubmed |
| pubmed-article:809280 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:809280 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:809280 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:809280 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:809280 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:809280 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:809280 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:809280 | pubmed:author | pubmed-author:PerssonUU | lld:pubmed |
| pubmed-article:809280 | pubmed:author | pubmed-author:PierceJJ | lld:pubmed |
| pubmed-article:809280 | pubmed:author | pubmed-author:ThulinEE | lld:pubmed |
| pubmed-article:809280 | pubmed:author | pubmed-author:LaurellC BCB | lld:pubmed |
| pubmed-article:809280 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:809280 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:809280 | pubmed:volume | 57 | lld:pubmed |
| pubmed-article:809280 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:809280 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:809280 | pubmed:pagination | 107-13 | lld:pubmed |
| pubmed-article:809280 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
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| pubmed-article:809280 | pubmed:meshHeading | pubmed-meshheading:809280-C... | lld:pubmed |
| pubmed-article:809280 | pubmed:year | 1975 | lld:pubmed |
| pubmed-article:809280 | pubmed:articleTitle | Purification of alpha1-antitrypsin from plasma through thiol-disulfide interchange. | lld:pubmed |
| pubmed-article:809280 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:809280 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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