Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:8083194rdf:typepubmed:Citationlld:pubmed
pubmed-article:8083194lifeskim:mentionsumls-concept:C1273518lld:lifeskim
pubmed-article:8083194lifeskim:mentionsumls-concept:C0034721lld:lifeskim
pubmed-article:8083194lifeskim:mentionsumls-concept:C0034693lld:lifeskim
pubmed-article:8083194lifeskim:mentionsumls-concept:C0227651lld:lifeskim
pubmed-article:8083194lifeskim:mentionsumls-concept:C0033551lld:lifeskim
pubmed-article:8083194lifeskim:mentionsumls-concept:C0017262lld:lifeskim
pubmed-article:8083194lifeskim:mentionsumls-concept:C0086982lld:lifeskim
pubmed-article:8083194lifeskim:mentionsumls-concept:C2911684lld:lifeskim
pubmed-article:8083194lifeskim:mentionsumls-concept:C0185117lld:lifeskim
pubmed-article:8083194pubmed:issue37lld:pubmed
pubmed-article:8083194pubmed:dateCreated1994-10-11lld:pubmed
pubmed-article:8083194pubmed:abstractTextProstaglandin G/H synthase (PGHS) is one of the key enzymes in prostaglandin synthesis. Regulation of the mRNA expression of the two isozymes PGHS-1 and PGHS-2 was investigated in mesangial cells. PGHS-1 was constitutively expressed and not modulated by any of the stimuli used. PGHS-2 was induced by the platelet products serotonin (5-HT) and thromboxane A2 (used as its analogue U46619), but not by ATP. Expression of PGHS protein was regulated correspondingly; whereas PGHS-1 protein was constitutively expressed, PGHS-2 protein was virtually absent in unstimulated cells, but could increasingly be induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), 5-HT, or fetal calf serum. Induction of PGHS-2 mRNA was transient with a peak after 2-3 h. Stimulated mRNA levels persisted for more than 6 h when transcription was inhibited by actinomycin D or when translation was inhibited by cycloheximide. As shown by specific inhibitors, 5-HT signal transduction was mediated by 5-HT2 receptors, which couple to phospholipase C via pertussis toxin-sensitive G-proteins. Induction of PGHS-2 mRNA by 5-HT was dependent on protein kinase C. Down-regulation of the enzyme by prolonged incubation with TPA abolished 5-HT-induced PGHS-2 mRNA expression. Short time activation of protein kinase C by TPA induced PGHS-2 mRNA expression. On the other hand, TPA given immediately before 5-HT decreased the 5-HT-induced PGHS-2 mRNA expression, indicating a negative feedback. The immunosuppressive drug cyclosporin A reduced induction of PGHS-2 mRNA expression by 5-HT, indicating interference with the signaling cascade, most likely with the Ser/Thr phosphatase calcineurin. Involvement of Tyr phosphorylation in 5-HT signaling was shown by the Tyr kinase inhibitor genistein, which inhibited the induction, while the Tyr phosphatase inhibitor vanadate by itself was able to induce PGHS-2 mRNA expression, which was further augmented when vanadate was combined with 5-HT. PGHS-2 mRNA expression is thus tightly regulated in mesangial cells and therefore allows modulation at various levels by physiological and pharmacological stimuli.lld:pubmed
pubmed-article:8083194pubmed:languageenglld:pubmed
pubmed-article:8083194pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:citationSubsetIMlld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8083194pubmed:statusMEDLINElld:pubmed
pubmed-article:8083194pubmed:monthSeplld:pubmed
pubmed-article:8083194pubmed:issn0021-9258lld:pubmed
pubmed-article:8083194pubmed:authorpubmed-author:Goppelt-Strue...lld:pubmed
pubmed-article:8083194pubmed:authorpubmed-author:StroebelMMlld:pubmed
pubmed-article:8083194pubmed:issnTypePrintlld:pubmed
pubmed-article:8083194pubmed:day16lld:pubmed
pubmed-article:8083194pubmed:volume269lld:pubmed
pubmed-article:8083194pubmed:ownerNLMlld:pubmed
pubmed-article:8083194pubmed:authorsCompleteYlld:pubmed
pubmed-article:8083194pubmed:pagination22952-7lld:pubmed
pubmed-article:8083194pubmed:dateRevised2007-11-15lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:meshHeadingpubmed-meshheading:8083194-...lld:pubmed
pubmed-article:8083194pubmed:year1994lld:pubmed
pubmed-article:8083194pubmed:articleTitleSignal transduction pathways responsible for serotonin-mediated prostaglandin G/H synthase expression in rat mesangial cells.lld:pubmed
pubmed-article:8083194pubmed:affiliationDepartment of Medicine IV, University of Erlangen-Nuernberg, Federal Republic of Germany.lld:pubmed
pubmed-article:8083194pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8083194pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8083194lld:pubmed