Linked Life Data

8062143

Source:http://linkedlifedata.com/resource/pubmed/id/8062143

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pubmed-article:8062143pubmed:abstractTextThe present study evaluates differential occurrence of voltage-dependent calcium channels (VDCC) in the membranes of fetal (FROB) and neonatal (NROB) calvarian rat osteoblastic cells in primary culture. The intracellular calcium concentration ([Ca2+]i) was monitored upon depolarization of the cell membrane with the use of high K+ containing extracellular solutions. [Ca2+]i was measured in populations of cells as well as in individual cells using Fura-2, whereas the membrane potential (Em) was recorded in parallel experiments using patch-clamp techniques. Increasing the extracellular K+ concentration resulted in an instantaneous depolarization of Em of both FROB and NROB. This depolarization of Em did not significantly affect [Ca2+]i of populations of FROB and neonatal osteoblast precursors (NpROB). In contrast to FROB and NpROB, NROB populations responded to depolarization with significant transient [Ca2+]i increases that could be blocked by the calcium antagonist verapamil and were absent if extracellular Na+ was replaced for choline instead of K+. In individual cell measurements, response frequencies as well as the magnitude of [Ca2+]i responses upon depolarization of NROB were much higher than those of FROB, suggesting that more NROB than FROB possess VDCC. This phenomenon might point to a development-related expression of VDCC in the membranes of osteoblast-like cells.lld:pubmed
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pubmed-article:8062143pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:8062143pubmed:year1994lld:pubmed
pubmed-article:8062143pubmed:articleTitleDifferential depolarization-activated calcium responses in fetal and neonatal rat osteoblast-like cells.lld:pubmed
pubmed-article:8062143pubmed:affiliationDepartment of Physiology and Physiological Physics, Leiden University, The Netherlands.lld:pubmed
pubmed-article:8062143pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8062143pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:8062143pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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