| pubmed-article:8050362 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C0282641 | lld:lifeskim |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C0001675 | lld:lifeskim |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C1522424 | lld:lifeskim |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C0017471 | lld:lifeskim |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C0018067 | lld:lifeskim |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C0039597 | lld:lifeskim |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C1257909 | lld:lifeskim |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:8050362 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:8050362 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:8050362 | pubmed:dateCreated | 1994-9-7 | lld:pubmed |
| pubmed-article:8050362 | pubmed:abstractText | The Zfy-1 and Zfy-2 genes, which arose by gene duplication, map to the mouse Y chromosome and encode nearly identical zinc-finger proteins. Zfy-1 is expressed in the genital ridge and adult testis and likely encodes a transcription activator. Although potential roles in sex determination and spermatogenesis have been hotly debated, the biological functions of Zfy-1 remain unknown. To study the gene's regulation, transgenes with 21-28 kb of Zfy-1 5' flanking DNA placed upstream of lacZ were constructed in plasmids or created by homologous recombination of coinjected DNA molecules. The resulting transgenic mice expressed beta-galactosidase in the genital ridge of both males and females starting between embryonic day 10 and 11 (E10-E11), peaking at E12-E13 and then declining to low levels by E15, a pattern that matches Zfy-1 mRNA as detected by RT-PCR. This lacZ expression in genital ridge was confined to somatic cells as demonstrated by its absence from the alkaline phosphatase-positive germ cells. It had been reported previously that Zfy-1 mRNA was absent from the embryonic gonad of homozygous W(e) embryos, which virtually lack germ cells. By contrast, we observed normal expression of the Zfy-1/lacZ transgene when introduced into the W(e) background, suggesting that germ cells are not necessary for expression. In the adult, the Zfy-1/lacZ transgene is expressed abundantly in developing germ cells. Extragonadal (kidney, meninges, arteries, choroid plexus) expression of the transgene was also observed in embryos. A smaller transgene with only 4.3 kb of Zfy-1 5' flanking DNA was expressed only in germ cells of adult mice. These results suggest that an enhancer for germ cell expression in the adult lies near the Zfy-1 promoter and that an enhancer for expression in the somatic cells of the embryonic gonad is located further 5'. | lld:pubmed |
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| pubmed-article:8050362 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8050362 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8050362 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8050362 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8050362 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8050362 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:8050362 | pubmed:issn | 0950-1991 | lld:pubmed |
| pubmed-article:8050362 | pubmed:author | pubmed-author:BrinsterR LRL | lld:pubmed |
| pubmed-article:8050362 | pubmed:author | pubmed-author:PalmiterR DRD | lld:pubmed |
| pubmed-article:8050362 | pubmed:author | pubmed-author:PageD CDC | lld:pubmed |
| pubmed-article:8050362 | pubmed:author | pubmed-author:SimpsonE MEM | lld:pubmed |
| pubmed-article:8050362 | pubmed:author | pubmed-author:ZimmermannJ... | lld:pubmed |
| pubmed-article:8050362 | pubmed:author | pubmed-author:HarendzaC JCJ | lld:pubmed |
| pubmed-article:8050362 | pubmed:author | pubmed-author:ZambrowiczB... | lld:pubmed |
| pubmed-article:8050362 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8050362 | pubmed:volume | 120 | lld:pubmed |
| pubmed-article:8050362 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8050362 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8050362 | pubmed:pagination | 1549-59 | lld:pubmed |
| pubmed-article:8050362 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8050362 | pubmed:meshHeading | pubmed-meshheading:8050362-... | lld:pubmed |
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| pubmed-article:8050362 | pubmed:meshHeading | pubmed-meshheading:8050362-... | lld:pubmed |
| pubmed-article:8050362 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8050362 | pubmed:articleTitle | Expression of a mouse Zfy-1/lacZ transgene in the somatic cells of the embryonic gonad and germ cells of the adult testis. | lld:pubmed |
| pubmed-article:8050362 | pubmed:affiliation | Howard Hughes Medical Institute, University of Washington SL-15, Seattle 98195. | lld:pubmed |
| pubmed-article:8050362 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8050362 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8050362 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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