| pubmed-article:8035807 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8035807 | lifeskim:mentions | umls-concept:C1420679 | lld:lifeskim |
| pubmed-article:8035807 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:8035807 | lifeskim:mentions | umls-concept:C2249858 | lld:lifeskim |
| pubmed-article:8035807 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:8035807 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:8035807 | pubmed:dateCreated | 1994-8-15 | lld:pubmed |
| pubmed-article:8035807 | pubmed:abstractText | Transcription in HeLa cell extracts in vitro was stimulated 8- to 10-fold by a recombinant chimera, GAL-TEF-1, consisting of the DNA-binding domain of GAL4 and the activation function of the HeLa cell activator TEF-1. In contrast, only a 2- to 3-fold stimulation was obtained with GAL-TEF-1 in extracts from BJA-B lymphoid cells. Stimulation by GAL-TEF-1 in BJA-B extracts was dramatically increased by the addition of immunopurified HeLa cell TFIID, suggesting that BJA-B TFIID lacks or contains lower quantities of a TATA-binding-protein-associated factor(s) required for the activity of the TEF-1 activation function. However, chromatography, immunopurification, and transcriptional reconstitution experiments indicated that BJA-B extracts did not lack the previously identified TATA-binding-protein-associated factors required for TEF-1 activity but rather contained a negatively acting factor(s) which inhibited transactivation by GAL-TEF-1. These results indicate that the relative lack of activity of the TEF-1 activation function in vitro in BJA-B cell extracts does not result from the absence of positively acting factors from the presence of a cell-specific negatively acting factor(s). | lld:pubmed |
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| pubmed-article:8035807 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8035807 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8035807 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8035807 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8035807 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8035807 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8035807 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:8035807 | pubmed:author | pubmed-author:ChambonPP | lld:pubmed |
| pubmed-article:8035807 | pubmed:author | pubmed-author:ChaudharySS | lld:pubmed |
| pubmed-article:8035807 | pubmed:author | pubmed-author:BroxLL | lld:pubmed |
| pubmed-article:8035807 | pubmed:author | pubmed-author:DavidsonII | lld:pubmed |
| pubmed-article:8035807 | pubmed:author | pubmed-author:BurtonNN | lld:pubmed |
| pubmed-article:8035807 | pubmed:author | pubmed-author:ToriTT | lld:pubmed |
| pubmed-article:8035807 | pubmed:author | pubmed-author:ValentinM EME | lld:pubmed |
| pubmed-article:8035807 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8035807 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:8035807 | pubmed:geneSymbol | GAL4 | lld:pubmed |
| pubmed-article:8035807 | pubmed:geneSymbol | TEF-1 | lld:pubmed |
