| pubmed-article:8021224 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8021224 | lifeskim:mentions | umls-concept:C0600492 | lld:lifeskim |
| pubmed-article:8021224 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8021224 | lifeskim:mentions | umls-concept:C0020284 | lld:lifeskim |
| pubmed-article:8021224 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:8021224 | lifeskim:mentions | umls-concept:C0062699 | lld:lifeskim |
| pubmed-article:8021224 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:8021224 | pubmed:dateCreated | 1994-8-4 | lld:pubmed |
| pubmed-article:8021224 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8021224 | pubmed:abstractText | Nucleotide sequence analysis revealed a 1,791-bp open reading frame in the hox gene cluster of the gram-negative chemolithotroph Alcaligenes eutrophus H16. In order to investigate the biological role of this open reading frame, we generated an in-frame deletion allele via a gene replacement strategy. The resulting mutant grew significantly more slowly than the wild type under lithoautotrophic conditions (6.1 versus 4.2 h doubling time). A reduction in the level of the soluble NAD-reducing hydrogenase (60% of the wild-type activity) was shown to be the cause of the slow lithoautotrophic growth. We used plasmid-borne gene fusions to monitor the expression of the operons encoding the soluble and membrane-bound hydrogenases. The expression of both operons was lower in the mutant than in the wild-type strain. These results suggest that the newly identified gene, designated hoxX, encodes a regulatory component which, in conjunction with the transcriptional activator HoxA, controls hydrogenase synthesis. | lld:pubmed |
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| pubmed-article:8021224 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8021224 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8021224 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8021224 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8021224 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8021224 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:8021224 | pubmed:author | pubmed-author:SchwartzEE | lld:pubmed |
| pubmed-article:8021224 | pubmed:author | pubmed-author:FriedrichBB | lld:pubmed |
| pubmed-article:8021224 | pubmed:author | pubmed-author:LeonRR | lld:pubmed |
| pubmed-article:8021224 | pubmed:author | pubmed-author:EitingerMM | lld:pubmed |
| pubmed-article:8021224 | pubmed:author | pubmed-author:DerneddeJJ | lld:pubmed |
| pubmed-article:8021224 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8021224 | pubmed:volume | 176 | lld:pubmed |
| pubmed-article:8021224 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8021224 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8021224 | pubmed:pagination | 4385-93 | lld:pubmed |
| pubmed-article:8021224 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:8021224 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8021224 | pubmed:articleTitle | The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation. | lld:pubmed |
| pubmed-article:8021224 | pubmed:affiliation | Institut für Pflanzenphysiologie und Mikrobiologie, Freien Universität Berlin, Germany. | lld:pubmed |
| pubmed-article:8021224 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8021224 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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