| pubmed-article:8015103 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8015103 | lifeskim:mentions | umls-concept:C0005682 | lld:lifeskim |
| pubmed-article:8015103 | lifeskim:mentions | umls-concept:C1517499 | lld:lifeskim |
| pubmed-article:8015103 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:8015103 | pubmed:issue | 2 Pt 1 | lld:pubmed |
| pubmed-article:8015103 | pubmed:dateCreated | 1994-7-28 | lld:pubmed |
| pubmed-article:8015103 | pubmed:abstractText | This study was designed to examine the potential for gene therapy in bladder in vivo using adenoviral vectors. Gene transfer to rat bladders was accomplished via direct intravesical instillation using a replication-defective adenoviral vector containing a marker gene encoding for Escherichia coli beta-galactosidase (beta-gal). Successful gene transfer was confirmed by analyzing bladder samples for DNA and RNA using polymerase chain reaction (PCR) with primers specific for beta-gal and adeno sequences, detecting beta-gal in full-thickness bladder wall using specific histochemical staining (X-gal) and documenting recombinant protein production. Bladder architecture was preserved, without evidence of distant spread of virus as assessed by PCR. Gene expression was evident for at least 7 days. In summary, bladder cells can be genetically altered using replication-deficient adenoviral vectors via simple intravesical instillation of vector. Introduction of exogenous genetic material is a potentially powerful therapeutic modality for immunomodulation of bladder neoplasms. | lld:pubmed |
| pubmed-article:8015103 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8015103 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8015103 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8015103 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:8015103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8015103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8015103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8015103 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8015103 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8015103 | pubmed:issn | 0022-5347 | lld:pubmed |
| pubmed-article:8015103 | pubmed:author | pubmed-author:ShakedAA | lld:pubmed |
| pubmed-article:8015103 | pubmed:author | pubmed-author:RosenthalJ... | lld:pubmed |
| pubmed-article:8015103 | pubmed:author | pubmed-author:CseteM EME | lld:pubmed |
| pubmed-article:8015103 | pubmed:author | pubmed-author:WerthmanP EPE | lld:pubmed |
| pubmed-article:8015103 | pubmed:author | pubmed-author:DrazanK EKE | lld:pubmed |
| pubmed-article:8015103 | pubmed:author | pubmed-author:MorrisB DBDJr | lld:pubmed |
| pubmed-article:8015103 | pubmed:author | pubmed-author:Van BreeM PMP | lld:pubmed |
| pubmed-article:8015103 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8015103 | pubmed:volume | 152 | lld:pubmed |
| pubmed-article:8015103 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8015103 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8015103 | pubmed:pagination | 506-9 | lld:pubmed |
| pubmed-article:8015103 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:meshHeading | pubmed-meshheading:8015103-... | lld:pubmed |
| pubmed-article:8015103 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:8015103 | pubmed:articleTitle | Adenoviral-mediated gene transfer to bladder in vivo. | lld:pubmed |
| pubmed-article:8015103 | pubmed:affiliation | Department of Surgery, University of California at Los Angeles 90024-6904. | lld:pubmed |
| pubmed-article:8015103 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8015103 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8015103 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8015103 | lld:pubmed |
