Linked Life Data

7992333

Source:http://linkedlifedata.com/resource/pubmed/id/7992333

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pubmed-article:7992333pubmed:abstractTextrK39 is a recombinant product of the 39 amino acid repeats found in a kinesin-like gene of visceral Leishmania spp. This and other antigens were compared for immunodiagnostic potential by enzyme-linked immunosorbent assay with sera from confirmed cases of Asian cutaneous and visceral leishmaniasis. In preliminary trials, rK39 proved superior to 2 purified Leishmania antigens, a cytosolic protein (p36) and a membrane protein (gp63), for immunodiagnosis of visceral leishmaniasis. Of the 53 visceral cases from China and Pakistan assayed, 52 were seropositive (98%) at a 10(-1) dilution with 36 ng of rK39. End point titrations of 27 highly positive samples yielded anti-rK39 antibody titres ranging from c. 10(-3) to beyond 10(-4). Antigen titrations with one positive serum further revealed that rK39 was 25-fold more sensitive than Leishmania whole cell soluble lysates. 31 cutaneous leishmaniasis cases from Turkey assayed for anti-rK39 antibody gave reactions ranging from negative or marginally positive to positive. In Brazil, all cutaneous and mucocutaneous leishmaniasis cases gave negative results in this assay.lld:pubmed
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pubmed-article:7992333pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:7992333pubmed:articleTitleSerodiagnosis of Asian leishmaniasis with a recombinant antigen from the repetitive domain of a Leishmania kinesin.lld:pubmed
pubmed-article:7992333pubmed:affiliationDepartment of Microbiology/Immunology, University of Health Sciences/Chicago Medical School, North Chicago, Illinois 60064.lld:pubmed
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