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| pubmed-article:7991578 | lifeskim:mentions | umls-concept:C0037633 | lld:lifeskim |
| pubmed-article:7991578 | lifeskim:mentions | umls-concept:C1706211 | lld:lifeskim |
| pubmed-article:7991578 | lifeskim:mentions | umls-concept:C0201699 | lld:lifeskim |
| pubmed-article:7991578 | lifeskim:mentions | umls-concept:C1148554 | lld:lifeskim |
| pubmed-article:7991578 | lifeskim:mentions | umls-concept:C1382100 | lld:lifeskim |
| pubmed-article:7991578 | lifeskim:mentions | umls-concept:C0007961 | lld:lifeskim |
| pubmed-article:7991578 | lifeskim:mentions | umls-concept:C1704419 | lld:lifeskim |
| pubmed-article:7991578 | lifeskim:mentions | umls-concept:C1546426 | lld:lifeskim |
| pubmed-article:7991578 | lifeskim:mentions | umls-concept:C1548280 | lld:lifeskim |
| pubmed-article:7991578 | pubmed:issue | 25 | lld:pubmed |
| pubmed-article:7991578 | pubmed:dateCreated | 1995-1-11 | lld:pubmed |
| pubmed-article:7991578 | pubmed:abstractText | This paper describes two methods to estimate the effective charge of a protein in solution by capillary electrophoresis and demonstrates these methods by using representative proteins. In one method, a "charge ladder"--a series of derivatives of a protein differing by known increments of charge but differing only minimally in hydrodynamic drag--is generated by covalent modification of the epsilon-amino groups of lysines with 4-sulfophenyl isothiocyanate or acetic anhydride. In the second method, the equivalent of a charge ladder is produced by noncovalent association of a protein with differently charged ligands. Analysis of the electrophoretic mobilities of the protein and its derivatives as a function of added charge allows the effective charge to be estimated for the unmodified protein. This type of analysis permits estimation of the effective charge of a protein without knowing its composition, structure, or amino acid sequence. | lld:pubmed |
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| pubmed-article:7991578 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:7991578 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7991578 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7991578 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:7991578 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:7991578 | pubmed:author | pubmed-author:WhitesidesG... | lld:pubmed |
| pubmed-article:7991578 | pubmed:author | pubmed-author:GalMM | lld:pubmed |
| pubmed-article:7991578 | pubmed:author | pubmed-author:HärterRR | lld:pubmed |
| pubmed-article:7991578 | pubmed:author | pubmed-author:GomezF AFA | lld:pubmed |
| pubmed-article:7991578 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7991578 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:7991578 | pubmed:volume | 91 | lld:pubmed |
| pubmed-article:7991578 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7991578 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7991578 | pubmed:pagination | 12027-30 | lld:pubmed |
| pubmed-article:7991578 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:7991578 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7991578 | pubmed:articleTitle | Determination of the effective charge of a protein in solution by capillary electrophoresis. | lld:pubmed |
| pubmed-article:7991578 | pubmed:affiliation | Department of Chemistry, Harvard University, Cambridge, MA 02138. | lld:pubmed |
| pubmed-article:7991578 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7991578 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:7991578 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7991578 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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