| pubmed-article:7986376 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7986376 | lifeskim:mentions | umls-concept:C0034861 | lld:lifeskim |
| pubmed-article:7986376 | lifeskim:mentions | umls-concept:C1512977 | lld:lifeskim |
| pubmed-article:7986376 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:7986376 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:7986376 | lifeskim:mentions | umls-concept:C1521871 | lld:lifeskim |
| pubmed-article:7986376 | lifeskim:mentions | umls-concept:C0449445 | lld:lifeskim |
| pubmed-article:7986376 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:7986376 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:7986376 | pubmed:dateCreated | 1995-1-9 | lld:pubmed |
| pubmed-article:7986376 | pubmed:abstractText | A method for the expression of recombinant DNA products in mammalian cells based on in vitro amplification of gene units is described. Gene cassettes containing either a selectable marker or the gene of interest are mixed at different molar ratios, and linear polymers are formed using forced head-to-tail ligation. After introduction of the polymers into mammalian cells, transformants with various amounts of the amplified gene unit are obtained. For a first characterization of the system, the gene coding for chloramphenicol acetyltransferase (CAT) has been used to produce polymers containing a single neomycin-resistance gene ligated to different numbers of CAT gene units and used for transfection into HeLa cells. All isolated G418 (gentamycin)-resistant cell transformants which received in vitro amplified DNA were found to express high levels of CAT activity in a stable manner. Southern-blot analysis of individual clones revealed multiple copies of the gene integrated head-to-tail in the genome. This system allowed us to express the gene coding for human prepro-endothelin-1 in A617 human vascular-smooth-muscle cells. The recombinant protein was shown to be correctly processed and biologically active endothelin-1. | lld:pubmed |
| pubmed-article:7986376 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7986376 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7986376 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7986376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7986376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7986376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7986376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7986376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7986376 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7986376 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7986376 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7986376 | pubmed:issn | 0885-4513 | lld:pubmed |
| pubmed-article:7986376 | pubmed:author | pubmed-author:SoriaM RMR | lld:pubmed |
| pubmed-article:7986376 | pubmed:author | pubmed-author:MonacoLL | lld:pubmed |
| pubmed-article:7986376 | pubmed:author | pubmed-author:TagliabueRR | lld:pubmed |
| pubmed-article:7986376 | pubmed:author | pubmed-author:UhlènMM | lld:pubmed |
| pubmed-article:7986376 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7986376 | pubmed:volume | 20 ( Pt 2) | lld:pubmed |
| pubmed-article:7986376 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7986376 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7986376 | pubmed:pagination | 157-71 | lld:pubmed |
| pubmed-article:7986376 | pubmed:dateRevised | 2007-3-21 | lld:pubmed |
| pubmed-article:7986376 | pubmed:meshHeading | pubmed-meshheading:7986376-... | lld:pubmed |
| pubmed-article:7986376 | pubmed:meshHeading | pubmed-meshheading:7986376-... | lld:pubmed |
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| pubmed-article:7986376 | pubmed:meshHeading | pubmed-meshheading:7986376-... | lld:pubmed |
| pubmed-article:7986376 | pubmed:meshHeading | pubmed-meshheading:7986376-... | lld:pubmed |
| pubmed-article:7986376 | pubmed:meshHeading | pubmed-meshheading:7986376-... | lld:pubmed |
| pubmed-article:7986376 | pubmed:meshHeading | pubmed-meshheading:7986376-... | lld:pubmed |
| pubmed-article:7986376 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7986376 | pubmed:articleTitle | An in vitro amplification approach for the expression of recombinant proteins in mammalian cells. | lld:pubmed |
| pubmed-article:7986376 | pubmed:affiliation | Department of Biological and Technological Research, San Raffaele Scientific Institute, Milan, Italy. | lld:pubmed |
| pubmed-article:7986376 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7986376 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
