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pubmed-article:7978246pubmed:abstractTextWe report the use of an artificial neural network to analyze the fingerprint region of Fourier-transform infrared (ir) spectra of oligosaccharides for the presence of sulfate groups. This assay can rapidly and nondestructively detect the presence of sulfate in as little as 1 nmol (approximately 2 micrograms) of a glycoprotein-derived monosulfated decasaccharide. The neural network was trained to recognize the presence of sulfate groups by presenting it with 45 ir spectra of sulfated and nonsulfated mono- and oligosaccharides. No prior knowledge of the characteristic ir spectral features of a sulfate group was needed as input. The training process required between 3 and 10 h, while analysis of a spectrum with the trained neural network requires only 0.1 s.lld:pubmed
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pubmed-article:7978246pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:7978246pubmed:year1994lld:pubmed
pubmed-article:7978246pubmed:articleTitleSulfate detection in glycoprotein-derived oligosaccharides by artificial neural network analysis of Fourier-transform infrared spectra.lld:pubmed
pubmed-article:7978246pubmed:affiliationComplex Carbohydrate Research Center, University of Georgia, Athens 30602-4712.lld:pubmed
pubmed-article:7978246pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7978246pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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