| pubmed-article:7966026 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C0001924 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C0017861 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C0038952 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C0016701 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C0037633 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C1382100 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C0439228 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C1548793 | lld:lifeskim |
| pubmed-article:7966026 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:7966026 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7966026 | pubmed:dateCreated | 1994-12-7 | lld:pubmed |
| pubmed-article:7966026 | pubmed:abstractText | A vitrification solution consisting of 6.5 mol glycerol l-1 and 6% (w/v) BSA in a modified Dulbecco's PBS (designated solution VS3a) was examined for the cryopreservation of 8-12-cell mouse embryos. Solution VS3a vitrified when cooled to -196 degrees C at rates of 10-2500 degrees C min-1 and vitrified suspensions did not crystallize when warmed at 200 or 2000 degrees C min-1. However, slow cooling at 5 degrees C min-1 or slow warming at 20 degrees C min-1 resulted in visible crystallization of solution VS3a. Embryos were equilibrated in solution VS3a in three steps at room temperature and placed into a 0.25 ml plastic straw in a way that permitted in-straw dilution with 1 mol sucrose l-1. Embryos equilibrated in solution VS3a and diluted immediately exhibited high rates of development in vitro to blastocysts (> 90%) if the total time of exposure to 100% solution VS3a did not exceed 5 min. Embryos exhibited high rates of development in vitro (75-97%) when equilibrated in 100% solution VS3a for 1 min and then cryopreserved using all combinations of three rates of cooling (5200 or 2500 degrees C min-1) and three rates of warming (20,000 or 2000 degrees C min-1). Although embryo suspensions visibly crystallized during slow cooling at 5 degrees C min-1, the rate of cooling was not a significant source of variance (P > 0.26). However, the rate of warming was found to have a small but significant effect on embryo survival (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:7966026 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7966026 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7966026 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7966026 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7966026 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7966026 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7966026 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7966026 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7966026 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:7966026 | pubmed:issn | 0022-4251 | lld:pubmed |
| pubmed-article:7966026 | pubmed:author | pubmed-author:WoodM JMJ | lld:pubmed |
| pubmed-article:7966026 | pubmed:author | pubmed-author:RallW FWF | lld:pubmed |
| pubmed-article:7966026 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7966026 | pubmed:volume | 101 | lld:pubmed |
| pubmed-article:7966026 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7966026 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7966026 | pubmed:pagination | 681-8 | lld:pubmed |
| pubmed-article:7966026 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:meshHeading | pubmed-meshheading:7966026-... | lld:pubmed |
| pubmed-article:7966026 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7966026 | pubmed:articleTitle | High in vitro and in vivo survival of day 3 mouse embryos vitrified or frozen in a non-toxic solution of glycerol and albumin. | lld:pubmed |
| pubmed-article:7966026 | pubmed:affiliation | Veterinary Resources Program, National Center for Research Resources, National Institutes of Health, Bethesda, MD 20892. | lld:pubmed |
| pubmed-article:7966026 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7966026 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7966026 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
