| pubmed-article:7957916 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C0815000 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C0034830 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C2003941 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:7957916 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:7957916 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:7957916 | pubmed:dateCreated | 1994-12-9 | lld:pubmed |
| pubmed-article:7957916 | pubmed:abstractText | Neuronal nicotinic acetylcholine receptors (nAChR) are made from different combinations of subunits encoded by a diverse family of genes. However, the recently cloned alpha 7 gene codes for subunits that can form homooligomeric nAChR complexes when expressed in Xenopus oocytes. Electrophysiological studies reveal that these alpha 7-nAChR function as alpha-bungarotoxin (Bgt)-sensitive, quickly activating/inactivating ion channels with a unique pharmacological profile and an unusually high permeability to calcium ions. Although similar observations have been made in studies of Bgt-sensitive, functional nAChR subtypes that are naturally expressed in neuronal cells, all attempts until now to reconstitute functional alpha 7-nAChR in cell lines have failed. Here we report the successful use of SH-SY5Y human neuroblastoma cells, which naturally express low levels of endogenous alpha 7 transcripts, to stably overexpress heterologous rat nAChR alpha 7 transgenes. These transgenes are expressed as the appropriately-sized alpha 7 messages and protein, and stably transfected SH-SY5Y cells have over 30-times higher levels of specific Bgt binding sites than do wild-type cells. Whole cell current recordings confirm that transfected cells express functional nAChR that are sensitive to blockade by Bgt and display the typical physiological and pharmacological profiles of alpha 7-nAChR. We conclude that stable, functional expression of alpha 7 transgenes in a mammalian cell line has been achieved for the first time. | lld:pubmed |
| pubmed-article:7957916 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7957916 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7957916 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7957916 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7957916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7957916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7957916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7957916 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7957916 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7957916 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7957916 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:7957916 | pubmed:author | pubmed-author:BertrandDD | lld:pubmed |
| pubmed-article:7957916 | pubmed:author | pubmed-author:LukasR JRJ | lld:pubmed |
| pubmed-article:7957916 | pubmed:author | pubmed-author:BuissonBB | lld:pubmed |
| pubmed-article:7957916 | pubmed:author | pubmed-author:PuchaczEE | lld:pubmed |
| pubmed-article:7957916 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7957916 | pubmed:day | 7 | lld:pubmed |
| pubmed-article:7957916 | pubmed:volume | 354 | lld:pubmed |
| pubmed-article:7957916 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7957916 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7957916 | pubmed:pagination | 155-9 | lld:pubmed |
| pubmed-article:7957916 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7957916 | pubmed:meshHeading | pubmed-meshheading:7957916-... | lld:pubmed |
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| pubmed-article:7957916 | pubmed:meshHeading | pubmed-meshheading:7957916-... | lld:pubmed |
| pubmed-article:7957916 | pubmed:meshHeading | pubmed-meshheading:7957916-... | lld:pubmed |
| pubmed-article:7957916 | pubmed:meshHeading | pubmed-meshheading:7957916-... | lld:pubmed |
| pubmed-article:7957916 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7957916 | pubmed:articleTitle | Functional expression of nicotinic acetylcholine receptors containing rat alpha 7 subunits in human SH-SY5Y neuroblastoma cells. | lld:pubmed |
| pubmed-article:7957916 | pubmed:affiliation | Division of Neurobiology, Barrow Neurological Institute, Phoenix, AZ 85013. | lld:pubmed |
| pubmed-article:7957916 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7957916 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7957916 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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