| pubmed-article:795580 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:795580 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:795580 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:795580 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:795580 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:795580 | lifeskim:mentions | umls-concept:C0004561 | lld:lifeskim |
| pubmed-article:795580 | lifeskim:mentions | umls-concept:C0444186 | lld:lifeskim |
| pubmed-article:795580 | lifeskim:mentions | umls-concept:C1947932 | lld:lifeskim |
| pubmed-article:795580 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:795580 | pubmed:dateCreated | 1977-3-31 | lld:pubmed |
| pubmed-article:795580 | pubmed:abstractText | A method enabling direct enumeration of mouse T and B cells from cytocentrifuged cell smears is described. The acid alpha-naphthyl acetate esterase (ANAE) staining was used as marker for T cells. The distribution of the ANAE marker-carrying lymphocytes closely followed the percentual distribution of T cells in spleen and lymph node. Electrophoresis analysis demonstrated that while more than 95% of resting spleen and lymph node T cells carried the ANAE marker, only less than 5% of the B cells were ANAE positive. The B cells (surface-bound Ig-carrying small lymphocytes) were identified by anti-Ig serum followed by rosetting of the B cells with Staphylococcus aureus strain Cowan 1 (StaCw). The ANAE marker-carrying cells did not overlap with the StaCw rosette-forming lymphocytes. Thus we conclude that the combination of StaCw rosetting and ANAE staining enables accurate identification of resting T and B cells from a single microscope slide. Ninety to 100% of Con A-activated blasts expressed the ANAE marker but only 60-85% of PHA and MLC-activated blasts were positive. Twenty to 33% of the blast cells stimulated by LPS expressed the ANAE marker. Thus the ANAE marker is not a reliable criterion for T cells in actibated state. | lld:pubmed |
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| pubmed-article:795580 | pubmed:language | eng | lld:pubmed |
| pubmed-article:795580 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:795580 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:795580 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:795580 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:795580 | pubmed:issn | 0009-9104 | lld:pubmed |
| pubmed-article:795580 | pubmed:author | pubmed-author:HäyryPP | lld:pubmed |
| pubmed-article:795580 | pubmed:author | pubmed-author:RankeEE | lld:pubmed |
| pubmed-article:795580 | pubmed:author | pubmed-author:TöttermanT... | lld:pubmed |
| pubmed-article:795580 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:795580 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:795580 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:795580 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:795580 | pubmed:pagination | 632-40 | lld:pubmed |
| pubmed-article:795580 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:795580 | pubmed:meshHeading | pubmed-meshheading:795580-R... | lld:pubmed |
| pubmed-article:795580 | pubmed:year | 1976 | lld:pubmed |
| pubmed-article:795580 | pubmed:articleTitle | Identification of mouse T and B lymphocytes from cytocentrifuged cell smears. | lld:pubmed |
| pubmed-article:795580 | pubmed:publicationType | Journal Article | lld:pubmed |
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