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pubmed-article:7951316pubmed:abstractTextWe have constructed a physical map of a 4 cM region on chromosome 17q12-21 that contains the hereditary breast and ovarian cancer gene BRCA1. The map comprises a contig of 137 overlapping yeast artificial chromosomes and P1 clones, onto which we have placed 112 PCR markers. We have localized more than 20 genes on this map, ten of which had not been mapped to the region previously, and have isolated 30 cDNA clones representing partial sequences of as yet unidentified genes. Two genes that lie within a narrow region defined by meiotic breakpoints in BRCA1 patients have been sequenced in breast cancer patients without revealing any deleterious mutations. These new reagents should facilitate the identification of BRCA1.lld:pubmed
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pubmed-article:7951316pubmed:articleTitleA physical map and candidate genes in the BRCA1 region on chromosome 17q12-21.lld:pubmed
pubmed-article:7951316pubmed:affiliationEccles Institute of Human Genetics, University of Utah, Salt Lake City 84112.lld:pubmed
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